Molecular interaction between lipoteichoic acids and Lactobacillus delbrueckii phages depends on D-alanyl and α-glucose substitution of poly(glycerophosphate) backbones

Liisa Räisänen, Christian Draing, Markus Pfitzenmaier, Karin Schubert, Tiina Jaakonsaari, Sonja Von Aulock, Thomas Hartung, Tapani Alatossava

Research output: Contribution to journalArticle

Abstract

Lipoteichoic acids (LTAs) have been shown to act as bacterial counterparts to the receptor binding proteins of LL-H, LL-H host range mutant LL-H-a21, and JCL1032. Here we have used LTAs purified by hydrophobic interaction chromatography from different phage-resistant and -sensitive strains of Lactobacillus delbrueckii subsp. lactis. Nuclear magnetic resonance analyses revealed variation in the degree of α-glucosyl and D-alanyl substitution of the 1,3-linked poly(glycerophosphate) LTAs between the phage-sensitive and phage-resistant strains. Inactivation of phages was less effective if there was a high level of D-alanine residues in the LTA backbones. Prior incubation of the LTAs with α-glucose-specific lectin inhibited the LL-H phage inactivation. The overall level of decoration or the specific spatial combination of α-glucosyl-substituted, D-alanyl-substituted, and nonsubstituted glycerol residues may also affect phage adsorption.

Original languageEnglish (US)
Pages (from-to)4135-4140
Number of pages6
JournalJournal of bacteriology
Volume189
Issue number11
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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