Molecular identification of an IgE-dependent histamine-releasing factor

Susan M. MacDonald, Thorunn Rafnar, Jacqueline Langdon, Lawrence M. Lichtenstein

Research output: Contribution to journalArticlepeer-review


An immunoglobulin E (IgE)-dependent histamine-releasing factor (HRF) produced by lymphocytes of atopic children and present in biological fluids of allergic patients has been identified and purified. Amino-terminal sequencing revealed extensive homology to a mouse protein, p21, and its human homolog, p23. Both recombinant proteins caused histamine release from the human basophils of a subpopulation of donors, and this release was dependent on IgE. Polyclonal antibodies recognized and removed the biological activity of recombinant and native HRF. HRF identifies a heterogeneity of IgE and is believed to play a prominent role in chronic allergic disease processes.

Original languageEnglish (US)
Pages (from-to)688-690
Number of pages3
Issue number5224
StatePublished - 1995

ASJC Scopus subject areas

  • General


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