TY - JOUR
T1 - Molecular genotyping of Mycobacterium tuberculosis in Xi'an, China, using MIRU-VNTR typing
AU - Zhou, A.
AU - Nawaz, M.
AU - Xue, X.
AU - Karakousis, P. C.
AU - Yao, Yufeng
AU - Xu, Jiru
PY - 2011/4
Y1 - 2011/4
N2 - SETTING: The molecular genotyping of Mycobacterium tuberculosis is expected to lead to a better understanding of M. tuberculosis transmission in Xi'an, one of the largest cities in Western China. OBJECTIVE: To characterize the population of M. tuberculosis strains circulating in Xi'an and to evaluate the feasibility of the mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing method. DESIGN: A total of 195 M. tuberculosis clinical isolates from Xi'an were genotyped using spoligotyping and MIRU-VNTR. RESULTS: Thirty-two different genotypes were identified by spoligotyping, with the Beijing family identified as the largest lineage (84.6%), followed by the T1 (3.08%), T2 (1.54%), H3 (1.03%) and U (1.03%) families. The Hunter-Gaston discriminatory index (HGDI) of spoligotyping is low (HGDI = 0.392). In contrast, 15-locus MIRU-VNTR shows a higher discriminatory power (HGDI = 0.998) than 12-locus MIRU-VNTR (HGDI = 0.948). By replacing the ETR C locus with the MIRU39 locus, the discriminatory power of 15-locus MIRU-VNTR is increased to 0.999. CONCLUSIONS: The Beijing family accounts for the vast majority of M. tuberculosis isolates in Xi'an, China. The modified 15-locus MIRU-VNTR showed high HGDI and can be used as a first-line genotyping method in combination with spoligotyping in routine epidemiological investigations in Xi'an, China.
AB - SETTING: The molecular genotyping of Mycobacterium tuberculosis is expected to lead to a better understanding of M. tuberculosis transmission in Xi'an, one of the largest cities in Western China. OBJECTIVE: To characterize the population of M. tuberculosis strains circulating in Xi'an and to evaluate the feasibility of the mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing method. DESIGN: A total of 195 M. tuberculosis clinical isolates from Xi'an were genotyped using spoligotyping and MIRU-VNTR. RESULTS: Thirty-two different genotypes were identified by spoligotyping, with the Beijing family identified as the largest lineage (84.6%), followed by the T1 (3.08%), T2 (1.54%), H3 (1.03%) and U (1.03%) families. The Hunter-Gaston discriminatory index (HGDI) of spoligotyping is low (HGDI = 0.392). In contrast, 15-locus MIRU-VNTR shows a higher discriminatory power (HGDI = 0.998) than 12-locus MIRU-VNTR (HGDI = 0.948). By replacing the ETR C locus with the MIRU39 locus, the discriminatory power of 15-locus MIRU-VNTR is increased to 0.999. CONCLUSIONS: The Beijing family accounts for the vast majority of M. tuberculosis isolates in Xi'an, China. The modified 15-locus MIRU-VNTR showed high HGDI and can be used as a first-line genotyping method in combination with spoligotyping in routine epidemiological investigations in Xi'an, China.
KW - MIRU-VNTR genotyping
KW - Molecular epidemiology
KW - Mycobacterium tuberculosis
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U2 - 10.5588/ijtld.10.0495
DO - 10.5588/ijtld.10.0495
M3 - Article
C2 - 21396212
AN - SCOPUS:79952795692
SN - 1027-3719
VL - 15
SP - 517
EP - 522
JO - International Journal of Tuberculosis and Lung Disease
JF - International Journal of Tuberculosis and Lung Disease
IS - 4
ER -