Molecular epidemiology of mycobacterium tuberculosis in Gansu province of China

Li li Tian, Hong yan Si, Tao jun Mu, Wen bing Fan, Jing Wang, Wei min Jiang, Qing Li, Biao Yang, Ying Zhang, Bing dong Zhu

Research output: Contribution to journalArticle

Abstract

Background Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) and Beijing family typing based on detecting the deletion of RD105 sequence are two common genotyping methods used to study the molecular epidemiologic characteristics of Mycobacterium (M.) tuberculosis. We collected 218 strains of M. tuberculosis between 2004 and 2006 in the Linxia Hui Autonomous Prefecture of Gansu province in Northwest China. Methods MIRU-VNTR analysis and Beijing family typing based on detecting the deletion of RD105 sequence were used to type the 218 strains, and their typing power was evaluated to look for practical and efficient genotyping methods suitable for the region. Results The MIRU typing yielded 115 distinct genotypes, including 98 unique isolates and 17 different clusters containing 120 isolates (55.05%); the cluster rate was 47.25%. By detecting the deletion of RD105 sequence, 188 of 218 (86.23%) isolates belonged to Beijing family. Combination of Beijing family typing and MIRU typing yielded 118 distinct patterns, including 101 unique isolates and 17 clusters containing 117 isolates (54.13%). The largest cluster contained 58 strains with MIRU genotype of 223325173533 which contained 50 strains belonging to Beijing family and 8 strains belonging to non-Beijing family. Conclusions The Beijing family strains occupied a large proportion and the Beijing family MIRU genotype 223325173533 is a dominant strain in Linxia of Gansu. Combining detecting the deletion of RD105 and MIRU typing together provides a simple, fast, and effective method which is low in cost and might be practical and suitable for M. tuberculosis genotyping in China.

Original languageEnglish (US)
Pages (from-to)3458-3464
Number of pages7
JournalChinese Medical Journal
Volume125
Issue number19
DOIs
StatePublished - Oct 5 2012

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Molecular Epidemiology
Mycobacterium tuberculosis
China
Sequence Deletion
Minisatellite Repeats
Genotype
Beijing
Epidemiologic Studies
Costs and Cost Analysis

Keywords

  • Beijing family
  • Epidemiology
  • Mycobacterial interspersed repetitive unit genotyping
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tian, L. L., Si, H. Y., Mu, T. J., Fan, W. B., Wang, J., Jiang, W. M., ... Zhu, B. D. (2012). Molecular epidemiology of mycobacterium tuberculosis in Gansu province of China. Chinese Medical Journal, 125(19), 3458-3464. https://doi.org/10.3760/cma.j.issn.0366-6999.2012.19.018

Molecular epidemiology of mycobacterium tuberculosis in Gansu province of China. / Tian, Li li; Si, Hong yan; Mu, Tao jun; Fan, Wen bing; Wang, Jing; Jiang, Wei min; Li, Qing; Yang, Biao; Zhang, Ying; Zhu, Bing dong.

In: Chinese Medical Journal, Vol. 125, No. 19, 05.10.2012, p. 3458-3464.

Research output: Contribution to journalArticle

Tian, LL, Si, HY, Mu, TJ, Fan, WB, Wang, J, Jiang, WM, Li, Q, Yang, B, Zhang, Y & Zhu, BD 2012, 'Molecular epidemiology of mycobacterium tuberculosis in Gansu province of China', Chinese Medical Journal, vol. 125, no. 19, pp. 3458-3464. https://doi.org/10.3760/cma.j.issn.0366-6999.2012.19.018
Tian, Li li ; Si, Hong yan ; Mu, Tao jun ; Fan, Wen bing ; Wang, Jing ; Jiang, Wei min ; Li, Qing ; Yang, Biao ; Zhang, Ying ; Zhu, Bing dong. / Molecular epidemiology of mycobacterium tuberculosis in Gansu province of China. In: Chinese Medical Journal. 2012 ; Vol. 125, No. 19. pp. 3458-3464.
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abstract = "Background Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) and Beijing family typing based on detecting the deletion of RD105 sequence are two common genotyping methods used to study the molecular epidemiologic characteristics of Mycobacterium (M.) tuberculosis. We collected 218 strains of M. tuberculosis between 2004 and 2006 in the Linxia Hui Autonomous Prefecture of Gansu province in Northwest China. Methods MIRU-VNTR analysis and Beijing family typing based on detecting the deletion of RD105 sequence were used to type the 218 strains, and their typing power was evaluated to look for practical and efficient genotyping methods suitable for the region. Results The MIRU typing yielded 115 distinct genotypes, including 98 unique isolates and 17 different clusters containing 120 isolates (55.05{\%}); the cluster rate was 47.25{\%}. By detecting the deletion of RD105 sequence, 188 of 218 (86.23{\%}) isolates belonged to Beijing family. Combination of Beijing family typing and MIRU typing yielded 118 distinct patterns, including 101 unique isolates and 17 clusters containing 117 isolates (54.13{\%}). The largest cluster contained 58 strains with MIRU genotype of 223325173533 which contained 50 strains belonging to Beijing family and 8 strains belonging to non-Beijing family. Conclusions The Beijing family strains occupied a large proportion and the Beijing family MIRU genotype 223325173533 is a dominant strain in Linxia of Gansu. Combining detecting the deletion of RD105 and MIRU typing together provides a simple, fast, and effective method which is low in cost and might be practical and suitable for M. tuberculosis genotyping in China.",
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N2 - Background Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) and Beijing family typing based on detecting the deletion of RD105 sequence are two common genotyping methods used to study the molecular epidemiologic characteristics of Mycobacterium (M.) tuberculosis. We collected 218 strains of M. tuberculosis between 2004 and 2006 in the Linxia Hui Autonomous Prefecture of Gansu province in Northwest China. Methods MIRU-VNTR analysis and Beijing family typing based on detecting the deletion of RD105 sequence were used to type the 218 strains, and their typing power was evaluated to look for practical and efficient genotyping methods suitable for the region. Results The MIRU typing yielded 115 distinct genotypes, including 98 unique isolates and 17 different clusters containing 120 isolates (55.05%); the cluster rate was 47.25%. By detecting the deletion of RD105 sequence, 188 of 218 (86.23%) isolates belonged to Beijing family. Combination of Beijing family typing and MIRU typing yielded 118 distinct patterns, including 101 unique isolates and 17 clusters containing 117 isolates (54.13%). The largest cluster contained 58 strains with MIRU genotype of 223325173533 which contained 50 strains belonging to Beijing family and 8 strains belonging to non-Beijing family. Conclusions The Beijing family strains occupied a large proportion and the Beijing family MIRU genotype 223325173533 is a dominant strain in Linxia of Gansu. Combining detecting the deletion of RD105 and MIRU typing together provides a simple, fast, and effective method which is low in cost and might be practical and suitable for M. tuberculosis genotyping in China.

AB - Background Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) and Beijing family typing based on detecting the deletion of RD105 sequence are two common genotyping methods used to study the molecular epidemiologic characteristics of Mycobacterium (M.) tuberculosis. We collected 218 strains of M. tuberculosis between 2004 and 2006 in the Linxia Hui Autonomous Prefecture of Gansu province in Northwest China. Methods MIRU-VNTR analysis and Beijing family typing based on detecting the deletion of RD105 sequence were used to type the 218 strains, and their typing power was evaluated to look for practical and efficient genotyping methods suitable for the region. Results The MIRU typing yielded 115 distinct genotypes, including 98 unique isolates and 17 different clusters containing 120 isolates (55.05%); the cluster rate was 47.25%. By detecting the deletion of RD105 sequence, 188 of 218 (86.23%) isolates belonged to Beijing family. Combination of Beijing family typing and MIRU typing yielded 118 distinct patterns, including 101 unique isolates and 17 clusters containing 117 isolates (54.13%). The largest cluster contained 58 strains with MIRU genotype of 223325173533 which contained 50 strains belonging to Beijing family and 8 strains belonging to non-Beijing family. Conclusions The Beijing family strains occupied a large proportion and the Beijing family MIRU genotype 223325173533 is a dominant strain in Linxia of Gansu. Combining detecting the deletion of RD105 and MIRU typing together provides a simple, fast, and effective method which is low in cost and might be practical and suitable for M. tuberculosis genotyping in China.

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