Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers

Yu Liu, Jingxing Wang, Yi Huang, Tonghan Yang, Xiaoming Guo, Julin Li, Guoxin Wen, Zhongqiao Yun, Peibin Zeng, Miao He, Min Xu, Gui Liu, Ling Ke, David Wright, Jing Liu, Kenrad Edwin Nelson, Hua Shan

Research output: Contribution to journalArticle

Abstract

Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 %) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 %, 21.9 %, 6.6 % and 3.95 % of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 %), adr (23.3 %) and ayw (8.7 %). Mutations were present in 39 (17.1 %) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.

Original languageEnglish (US)
Pages (from-to)1699-1707
Number of pages9
JournalArchives of Virology
Volume157
Issue number9
DOIs
StatePublished - Sep 2012

Fingerprint

Blood Donors
Hepatitis B virus
Epidemiologic Studies
Genotype
Hepatitis B Surface Antigens
Mutation
Hepatitis B Antigens
Amino Acid Sequence
Immunization
China
Safety

ASJC Scopus subject areas

  • Virology

Cite this

Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers. / Liu, Yu; Wang, Jingxing; Huang, Yi; Yang, Tonghan; Guo, Xiaoming; Li, Julin; Wen, Guoxin; Yun, Zhongqiao; Zeng, Peibin; He, Miao; Xu, Min; Liu, Gui; Ke, Ling; Wright, David; Liu, Jing; Nelson, Kenrad Edwin; Shan, Hua.

In: Archives of Virology, Vol. 157, No. 9, 09.2012, p. 1699-1707.

Research output: Contribution to journalArticle

Liu, Y, Wang, J, Huang, Y, Yang, T, Guo, X, Li, J, Wen, G, Yun, Z, Zeng, P, He, M, Xu, M, Liu, G, Ke, L, Wright, D, Liu, J, Nelson, KE & Shan, H 2012, 'Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers', Archives of Virology, vol. 157, no. 9, pp. 1699-1707. https://doi.org/10.1007/s00705-012-1331-x
Liu, Yu ; Wang, Jingxing ; Huang, Yi ; Yang, Tonghan ; Guo, Xiaoming ; Li, Julin ; Wen, Guoxin ; Yun, Zhongqiao ; Zeng, Peibin ; He, Miao ; Xu, Min ; Liu, Gui ; Ke, Ling ; Wright, David ; Liu, Jing ; Nelson, Kenrad Edwin ; Shan, Hua. / Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers. In: Archives of Virology. 2012 ; Vol. 157, No. 9. pp. 1699-1707.
@article{bc84893fb9e94fac91fb33449ce1bc32,
title = "Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers",
abstract = "Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 {\%}) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 {\%}, 21.9 {\%}, 6.6 {\%} and 3.95 {\%} of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 {\%}), adr (23.3 {\%}) and ayw (8.7 {\%}). Mutations were present in 39 (17.1 {\%}) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.",
author = "Yu Liu and Jingxing Wang and Yi Huang and Tonghan Yang and Xiaoming Guo and Julin Li and Guoxin Wen and Zhongqiao Yun and Peibin Zeng and Miao He and Min Xu and Gui Liu and Ling Ke and David Wright and Jing Liu and Nelson, {Kenrad Edwin} and Hua Shan",
year = "2012",
month = "9",
doi = "10.1007/s00705-012-1331-x",
language = "English (US)",
volume = "157",
pages = "1699--1707",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "9",

}

TY - JOUR

T1 - Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers

AU - Liu, Yu

AU - Wang, Jingxing

AU - Huang, Yi

AU - Yang, Tonghan

AU - Guo, Xiaoming

AU - Li, Julin

AU - Wen, Guoxin

AU - Yun, Zhongqiao

AU - Zeng, Peibin

AU - He, Miao

AU - Xu, Min

AU - Liu, Gui

AU - Ke, Ling

AU - Wright, David

AU - Liu, Jing

AU - Nelson, Kenrad Edwin

AU - Shan, Hua

PY - 2012/9

Y1 - 2012/9

N2 - Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 %) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 %, 21.9 %, 6.6 % and 3.95 % of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 %), adr (23.3 %) and ayw (8.7 %). Mutations were present in 39 (17.1 %) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.

AB - Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 %) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 %, 21.9 %, 6.6 % and 3.95 % of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 %), adr (23.3 %) and ayw (8.7 %). Mutations were present in 39 (17.1 %) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.

UR - http://www.scopus.com/inward/record.url?scp=84865748677&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865748677&partnerID=8YFLogxK

U2 - 10.1007/s00705-012-1331-x

DO - 10.1007/s00705-012-1331-x

M3 - Article

C2 - 22669316

AN - SCOPUS:84865748677

VL - 157

SP - 1699

EP - 1707

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 9

ER -