Molecular effects of genistein on male urethral development

Ashley E. Ross, Luigi Marchionni, Timothy M. Phillips, Rebecca M. Miller, Paula Hurley, Brian W. Simons, Amirali H. Salmasi, Anthony J. Schaeffer, John Phillip Gearhart, Edward M. Schaeffer

Research output: Contribution to journalArticle

Abstract

Purpose: The increasing incidence of hypospadias is partly attributed to increased gestational exposure to endocrine disruptors. We investigated the effects of genistein, the primary phytoestrogen in soy, on the molecular program of male urethral development. Materials and Methods: Female mice were fed diets supplemented with genistein (500 mg/kg diet) or control diets before breeding and throughout gestation. Urethras from embryonic day 17.5 male fetuses were harvested, and RNA was prepared, amplified, labeled and hybridized on whole genome microarrays. Data were analyzed using packages from the R/Bioconductor project. Immunohistochemical analysis and immunoblotting were used to confirm the activity of MAPK and the presence of Ntrk1 and Ntrk2 during urethral development. Results: Gestational exposure to genistein altered the urethral expression of 277 genes (p

Original languageEnglish (US)
Pages (from-to)1894-1898
Number of pages5
JournalJournal of Urology
Volume185
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Genistein
Diet
Endocrine Disruptors
Phytoestrogens
Hypospadias
Urethra
Immunoblotting
Breeding
Fetus
Genome
RNA
Gene Expression
Pregnancy
Incidence

Keywords

  • endocrine disruptors
  • gene expression profiling
  • genistein
  • hypospadias

ASJC Scopus subject areas

  • Urology

Cite this

Ross, A. E., Marchionni, L., Phillips, T. M., Miller, R. M., Hurley, P., Simons, B. W., ... Schaeffer, E. M. (2011). Molecular effects of genistein on male urethral development. Journal of Urology, 185(5), 1894-1898. https://doi.org/10.1016/j.juro.2010.12.095

Molecular effects of genistein on male urethral development. / Ross, Ashley E.; Marchionni, Luigi; Phillips, Timothy M.; Miller, Rebecca M.; Hurley, Paula; Simons, Brian W.; Salmasi, Amirali H.; Schaeffer, Anthony J.; Gearhart, John Phillip; Schaeffer, Edward M.

In: Journal of Urology, Vol. 185, No. 5, 05.2011, p. 1894-1898.

Research output: Contribution to journalArticle

Ross, AE, Marchionni, L, Phillips, TM, Miller, RM, Hurley, P, Simons, BW, Salmasi, AH, Schaeffer, AJ, Gearhart, JP & Schaeffer, EM 2011, 'Molecular effects of genistein on male urethral development', Journal of Urology, vol. 185, no. 5, pp. 1894-1898. https://doi.org/10.1016/j.juro.2010.12.095
Ross, Ashley E. ; Marchionni, Luigi ; Phillips, Timothy M. ; Miller, Rebecca M. ; Hurley, Paula ; Simons, Brian W. ; Salmasi, Amirali H. ; Schaeffer, Anthony J. ; Gearhart, John Phillip ; Schaeffer, Edward M. / Molecular effects of genistein on male urethral development. In: Journal of Urology. 2011 ; Vol. 185, No. 5. pp. 1894-1898.
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