Molecular determinants for cellular uptake of Tat protein of human immunodeficiency virus type 1 in brain cells

M. Ma, A. Nath

Research output: Contribution to journalArticle

Abstract

We measured the cellular uptake of 125I-labeled full-length Tat (amino acids 1 to 86) (125I-Tat1-86) and 125I-Tat1-72 (first exon) in human fetal astrocytes, neuroblastoma cells, and human fetal neurons and demonstrated that the uptake of 125I-Tat1-72 without the second exon was much lower than that of 125I-Tat1-86 (P <0.01). This suggests an important role for the C-terminal region of Tat for its cellular uptake. 125I-Tat uptake could be inhibited by dextran sulfate and competitively inhibited by unlabeled Tat but not by overlapping 15-mer peptides, suggesting that Tat internalization is charge and conformationally dependent. Interestingly, one of 15-mer peptides, Tat28-42, greatly enhanced 125I-Tat uptake. These findings are important for understanding the neuropathogenesis of human immunodeficiency virus type 1 infection and in the potential application of Tat for drug delivery to cells.

Original languageEnglish (US)
Pages (from-to)2495-2499
Number of pages5
JournalJournal of Virology
Volume71
Issue number3
StatePublished - 1997
Externally publishedYes

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tat Gene Products
Human immunodeficiency virus 1
HIV-1
Exons
uptake mechanisms
brain
Dextran Sulfate
Peptides
Brain
Virus Diseases
Neuroblastoma
Astrocytes
proteins
exons
cells
Neurons
Amino Acids
peptides
drug delivery systems
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular determinants for cellular uptake of Tat protein of human immunodeficiency virus type 1 in brain cells. / Ma, M.; Nath, A.

In: Journal of Virology, Vol. 71, No. 3, 1997, p. 2495-2499.

Research output: Contribution to journalArticle

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