Molecular detection of primary bladder cancer by microsatellite analysis

Li Mao, Mark P. Schoenberg, Marshall Scicchitano, Yener S. Erozan, Adrian Merlo, Donna Schwab, David Sidransky

Research output: Contribution to journalArticle

Abstract

Microsatellite DNA markers have been widely used as a tool for the detection of loss of heterozygosity and genomic instability in primary tumors. In a blinded study, urine samples from 25 patients with suspicious bladder lesions that had been identified cystoscopically were analyzed by this molecular method and by conventional cytology, Microsatellite changes matching those in the tumor were detected in the urine sediment of 19 of the 20 patients (95 percent) who were diagnosed with bladder cancer, whereas urine cytology detected cancer cells in 9 of 18 (50 percent) of the samples. These results suggest that microsatellite analysis, which in principle can be performed at about one-third the cost of cytology, may be a useful addition to current screening methods for detecting bladder cancer.

Original languageEnglish (US)
Pages (from-to)659-662
Number of pages4
JournalScience
Volume271
Issue number5249
DOIs
StatePublished - Feb 2 1996

ASJC Scopus subject areas

  • General

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    Mao, L., Schoenberg, M. P., Scicchitano, M., Erozan, Y. S., Merlo, A., Schwab, D., & Sidransky, D. (1996). Molecular detection of primary bladder cancer by microsatellite analysis. Science, 271(5249), 659-662. https://doi.org/10.1126/science.271.5249.659