Abstract
Smoking is directly responsible for approximately 90% of lung cancers and is also strongly associated with cancers of the head and neck, esophagus and urinary bladder. Our growing understanding of the molecular changes that underlie cancer progression has contributed to the development of novel molecular approaches for the detection of cancer. In this study, we review a number of recent studies that have used molecular techniques to detect neoplastic DNA from lung, head and neck, esophagus and bladder cancer. The majority of these approaches are based on polymerase chain reaction (PCR) based assays. These PCR-based techniques can detect a few clonal cancer cells containing a specific DNA mutation, microsatellite alteration, or CpG island methylation among an excess background of normal cells. The ability to accurately detect a small number of malignant cells in a wide range of clinical specimens including sputum, saliva, bronchoalveolar lavage fluid, urine, serum, plasma, or tissue has significant implications for screening high-risk individuals (such as cigarette smokers) for cancer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7289-7297 |
| Number of pages | 9 |
| Journal | Oncogene |
| Volume | 21 |
| Issue number | 48 REV. ISS. 6 |
| DOIs | |
| State | Published - Oct 21 2002 |
| Externally published | Yes |
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Keywords
- Methylation
- Microsatellite instability
- Molecular detection
- Mutation
- PCR
- Smoking
- Tumor
ASJC Scopus subject areas
- Molecular Biology
- Cancer Research
- Genetics
Cite this
Molecular detection approaches for smoking associated tumors. / Hu, Ying Chuan; Sidransky, David; Ahrendt, Steven A.
In: Oncogene, Vol. 21, No. 48 REV. ISS. 6, 21.10.2002, p. 7289-7297.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular detection approaches for smoking associated tumors
AU - Hu, Ying Chuan
AU - Sidransky, David
AU - Ahrendt, Steven A.
PY - 2002/10/21
Y1 - 2002/10/21
N2 - Smoking is directly responsible for approximately 90% of lung cancers and is also strongly associated with cancers of the head and neck, esophagus and urinary bladder. Our growing understanding of the molecular changes that underlie cancer progression has contributed to the development of novel molecular approaches for the detection of cancer. In this study, we review a number of recent studies that have used molecular techniques to detect neoplastic DNA from lung, head and neck, esophagus and bladder cancer. The majority of these approaches are based on polymerase chain reaction (PCR) based assays. These PCR-based techniques can detect a few clonal cancer cells containing a specific DNA mutation, microsatellite alteration, or CpG island methylation among an excess background of normal cells. The ability to accurately detect a small number of malignant cells in a wide range of clinical specimens including sputum, saliva, bronchoalveolar lavage fluid, urine, serum, plasma, or tissue has significant implications for screening high-risk individuals (such as cigarette smokers) for cancer.
AB - Smoking is directly responsible for approximately 90% of lung cancers and is also strongly associated with cancers of the head and neck, esophagus and urinary bladder. Our growing understanding of the molecular changes that underlie cancer progression has contributed to the development of novel molecular approaches for the detection of cancer. In this study, we review a number of recent studies that have used molecular techniques to detect neoplastic DNA from lung, head and neck, esophagus and bladder cancer. The majority of these approaches are based on polymerase chain reaction (PCR) based assays. These PCR-based techniques can detect a few clonal cancer cells containing a specific DNA mutation, microsatellite alteration, or CpG island methylation among an excess background of normal cells. The ability to accurately detect a small number of malignant cells in a wide range of clinical specimens including sputum, saliva, bronchoalveolar lavage fluid, urine, serum, plasma, or tissue has significant implications for screening high-risk individuals (such as cigarette smokers) for cancer.
KW - Methylation
KW - Microsatellite instability
KW - Molecular detection
KW - Mutation
KW - PCR
KW - Smoking
KW - Tumor
UR - http://www.scopus.com/inward/record.url?scp=0037152653&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037152653&partnerID=8YFLogxK
U2 - 10.1038/sj.onc.1205805
DO - 10.1038/sj.onc.1205805
M3 - Article
C2 - 12379873
AN - SCOPUS:0037152653
VL - 21
SP - 7289
EP - 7297
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 48 REV. ISS. 6
ER -