Molecular detection and characterization of sapovirus in hospitalized children with acute gastroenteritis in the Philippines

Xiaofang Liu, Dai Yamamoto, Mariko Saito, Toshifumi Imagawa, Adrianne Ablola, Amado O. Tandoc, Edelwisa Segubre-Mercado, Socorro P. Lupisan, Michiko Okamoto, Yuki Furuse, Mayuko Saito, Hitoshi Oshitani

Research output: Contribution to journalArticle

Abstract

Background: Human sapovirus (SaV) is a causative agent of acute gastroenteritis. Recently, SaV detection has been increasing worldwide due to the emerging SaV genotype I.2. However, SaV infection has not been reported in the Philippines. Objectives: To evaluate the prevalence and genetic diversity of SaV in hospitalized children aged less than 5 years with acute gastroenteritis. Study design: Stool samples were collected from children with acute gastroenteritis at three hospitals in the Philippines from June 2012 to August 2013. SaV was detected by reverse transcription real-time PCR, and the polymerase and capsid gene sequences were analyzed. Full genome sequencing and recombination analysis were performed on possible recombinant viruses. Results: SaV was detected in 7.0% of the tested stool samples (29/417). In 10 SaV-positive cases, other viruses were also detected, including rotavirus (n=6), norovirus (n=2), and human astrovirus (n=2). Four known SaV genotypes (GI.1 [7], GI.2 [2], GII.1 [12], and GV [2]) and one novel recombinant (n=3) were identified by polymerase and capsid gene sequence analysis. Full genome sequencing revealed that the 5[U+02B9] nontranslated region (NTR) and nonstructural protein region of the novel recombinant were closely related to the GII.1 Bristol/98/UK variant, whereas the structural protein region and 3[U+02B9] NTR were closely related to the GII.4 Kumamoto6/Mar2003/JPN variant. Discussion and conclusions: SaV was regularly detected in hospitalized children due to acute gastroenteritis during the study period. A novel recombinant, SaV GII.1/GII.4, was identified in three cases at two different study sites.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalJournal of Clinical Virology
Volume68
DOIs
StatePublished - Jul 1 2015
Externally publishedYes

Keywords

  • Gastroenteritis
  • Recombination
  • Sapovirus
  • The Philippines

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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  • Cite this

    Liu, X., Yamamoto, D., Saito, M., Imagawa, T., Ablola, A., Tandoc, A. O., Segubre-Mercado, E., Lupisan, S. P., Okamoto, M., Furuse, Y., Saito, M., & Oshitani, H. (2015). Molecular detection and characterization of sapovirus in hospitalized children with acute gastroenteritis in the Philippines. Journal of Clinical Virology, 68, 83-88. https://doi.org/10.1016/j.jcv.2015.05.001