Molecular detection and analysis of spotted fever group Rickettsia in patients with fever and rash at a tertiary care centre in Tamil Nadu, India

John Antony Jude Prakash, T. Sohan Lal, Varghese Rosemol, Valsan Philip Verghese, Susanne A. Pulimood, Megan Reller, John Stephen Dumler

Research output: Contribution to journalArticle

Abstract

Background: Detection of specific targets by PCR is used to confirm a diagnosis of spotted fever, but serological tests are still widely used. In this prospective study, nested PCR was performed on skin biopsy specimens to confirm the diagnosis of spotted fever. Methods: In 58 clinically suspected cases of spotted fever, nested PCR, to detect gltA, 17 kDa lipoprotein antigen gene (17 kDa), ompA and ompB, from skin biopsy of the rash was performed. Sequencing was carried on amplicons representing the four targets to confirm specificity of amplification. This was followed by phylogenetic analysis using MEGA version 4.0 software. Results: The gltA, 17 kDa, ompA, and ompB genes were detected from skin biopsy specimens in 38, 23, 27, and 22 individuals. Sequence analysis revealed that the gltA, 17 kDa, ompA, and ompB sequences belonged to spotted fever group (SFG) rickettsia. Of the six partial ompA gene sequences, only one was dissimilar to the previously reported 'Candidatus Rickettsia kellyi'. Conclusion: Further evidence indicates that SFG rickettsiae resembling 'Candidatus Rickettsia kellyi' cause fever and rash in southern India. More detailed phylogenetic analysis following isolation of rickettsia in culture is required for providing irrefutable proof for the occurrence of novel spotted fever rickettsiae in this region.

Original languageEnglish (US)
Pages (from-to)40-45
Number of pages6
JournalPathogens and Global Health
Volume106
Issue number1
DOIs
StatePublished - Mar 2012

Fingerprint

Rickettsia
Exanthema
Tertiary Care Centers
India
Fever
Biopsy
Polymerase Chain Reaction
Genes
Skin
Serologic Tests
Lipoproteins
Sequence Analysis
Software
Prospective Studies
Antigens

Keywords

  • Fever with rash
  • Nested PCR
  • OmpA gene
  • Phylogeny
  • Skin biopsy
  • Spotted fever group (SFG) rickettsia

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Microbiology

Cite this

Molecular detection and analysis of spotted fever group Rickettsia in patients with fever and rash at a tertiary care centre in Tamil Nadu, India. / Prakash, John Antony Jude; Sohan Lal, T.; Rosemol, Varghese; Verghese, Valsan Philip; Pulimood, Susanne A.; Reller, Megan; Dumler, John Stephen.

In: Pathogens and Global Health, Vol. 106, No. 1, 03.2012, p. 40-45.

Research output: Contribution to journalArticle

Prakash, John Antony Jude ; Sohan Lal, T. ; Rosemol, Varghese ; Verghese, Valsan Philip ; Pulimood, Susanne A. ; Reller, Megan ; Dumler, John Stephen. / Molecular detection and analysis of spotted fever group Rickettsia in patients with fever and rash at a tertiary care centre in Tamil Nadu, India. In: Pathogens and Global Health. 2012 ; Vol. 106, No. 1. pp. 40-45.
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