Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification

Michael R. Speicher; Stanislas Du Manoir; Evelin Schröck; Heidi Holtgreve-Grez; Brigitte Schoell; Christoph Lengauer; Thomas Cremer; Thomas Ried

Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification

Michael R. Speicher, Stanislas Du Manoir, Evelin Schröck, Heidi Holtgreve-Grez, Brigitte Schoell, Christoph Lengauer, Thomas Cremer, Thomas Ried

Research output: Contribution to journal › Article › peer-review

161 Scopus citations

Abstract

We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications in archived solid tumor samples.

Original language	English (US)
Pages (from-to)	1907-1914
Number of pages	8
Journal	Human Molecular Genetics
Volume	2
Issue number	11
State	Published - Nov 1993
Externally published	Yes

ASJC Scopus subject areas

Genetics
Statistics, Probability and Uncertainty
Applied Mathematics
Public Health, Environmental and Occupational Health
Molecular Biology
Genetics(clinical)

Cite this

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abstract = "We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications in archived solid tumor samples.",

author = "Speicher, {Michael R.} and {Du Manoir}, Stanislas and Evelin Schr{\"o}ck and Heidi Holtgreve-Grez and Brigitte Schoell and Christoph Lengauer and Thomas Cremer and Thomas Ried",

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AU - Speicher, Michael R.

AU - Du Manoir, Stanislas

AU - Schröck, Evelin

AU - Holtgreve-Grez, Heidi

AU - Schoell, Brigitte

AU - Lengauer, Christoph

AU - Cremer, Thomas

AU - Ried, Thomas

PY - 1993/11

Y1 - 1993/11

N2 - We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications in archived solid tumor samples.

AB - We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications in archived solid tumor samples.

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ER -

Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification

Abstract

ASJC Scopus subject areas

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