Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification

Michael R. Speicher, Stanislas Du Manoir, Evelin Schröck, Heidi Holtgreve-Grez, Brigitte Schoell, Christoph Lengauer, Thomas Cremer, Thomas Ried

Research output: Contribution to journalArticle

Abstract

We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications in archived solid tumor samples.

Original languageEnglish (US)
Pages (from-to)1907-1914
Number of pages8
JournalHuman Molecular Genetics
Volume2
Issue number11
StatePublished - Nov 1993
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)

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  • Cite this

    Speicher, M. R., Du Manoir, S., Schröck, E., Holtgreve-Grez, H., Schoell, B., Lengauer, C., Cremer, T., & Ried, T. (1993). Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification. Human Molecular Genetics, 2(11), 1907-1914.