Molecular confirmation of human immunodeficiency virus (HIV) type 2 in HIV-seropositive subjects in south India

R. Kannangai, S. Ramalingam, K. J. Prakash, O. C. Abraham, R. George, Renan Carlos Castillo, D. H. Schwartz, M. V. Jesudason, G. Sridharan

Research output: Contribution to journalArticle

Abstract

Nested PCRs for human immunodeficiency virus type 1 (HIV-1) and HIV-2 were compared with immunoblot test results. Twelve of 13 immunoblot-positive HIV-2 samples were positive by PCR. There were five INNO-LIA (Innogenetics, Zwijnaarde, Belgium) and/or HIVBLOT 2.2 (Genelabs, Singapore) samples that tested positive for dual infection. HIV-1 PCR was positive in all samples, while HIV-2 PCR was positive in two and RIBA (Chiron Corporation, San Diego, Calif.) was positive for HIV-2 in three samples. Thus the prevalence of HIV-2 is accurately estimated by the use of immunoblotting, but that of HIV-1 and -2 dual infection may be overestimated.

Original languageEnglish (US)
Pages (from-to)987-989
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume7
Issue number6
DOIs
StatePublished - 2000
Externally publishedYes

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HIV-2
Viruses
India
HIV
HIV-1
Polymerase Chain Reaction
Belgium
Singapore
Infection
Immunoblotting

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Molecular confirmation of human immunodeficiency virus (HIV) type 2 in HIV-seropositive subjects in south India. / Kannangai, R.; Ramalingam, S.; Prakash, K. J.; Abraham, O. C.; George, R.; Castillo, Renan Carlos; Schwartz, D. H.; Jesudason, M. V.; Sridharan, G.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 7, No. 6, 2000, p. 987-989.

Research output: Contribution to journalArticle

Kannangai, R, Ramalingam, S, Prakash, KJ, Abraham, OC, George, R, Castillo, RC, Schwartz, DH, Jesudason, MV & Sridharan, G 2000, 'Molecular confirmation of human immunodeficiency virus (HIV) type 2 in HIV-seropositive subjects in south India', Clinical and Diagnostic Laboratory Immunology, vol. 7, no. 6, pp. 987-989. https://doi.org/10.1128/CDLI.7.6.987-989.2000
Kannangai, R. ; Ramalingam, S. ; Prakash, K. J. ; Abraham, O. C. ; George, R. ; Castillo, Renan Carlos ; Schwartz, D. H. ; Jesudason, M. V. ; Sridharan, G. / Molecular confirmation of human immunodeficiency virus (HIV) type 2 in HIV-seropositive subjects in south India. In: Clinical and Diagnostic Laboratory Immunology. 2000 ; Vol. 7, No. 6. pp. 987-989.
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