Molecular cloning, sequence analysis and mRNA expression of human ADP-ribosylation factor

Z. Peng, I. Calvert, J. Clark, L. Helman, R. Kahn, H. F. Kung

Research output: Contribution to journalArticlepeer-review

Abstract

The ADP-ribosylation factor (ARF) is the small (21 kb) GTP-binding protein required for the efficient cholera toxin-catalyzed ADP-ribosylation of purified Gs, the stimulating regulatory component of adenylate cyclase. Human ARF cDNA clones were obtained from a human cDNA library by cross-species hybridization with bovine ARF1, and the nucleotide and deduced amino acid sequences were determined. Comparison of the sequences of human and bovine ARF1 showed 90% identity at the nucleotide level and 100% identity at the amino acid level, demonstrating the highly conserved nature of the ARF protein. Using human ARF cDNA as the probe, we have detected ARF messenger RNA (~2.2-2.3 kb) in a wide variety of human tissues and tumor cell lines.

Original languageEnglish (US)
Pages (from-to)45-49
Number of pages5
JournalBioFactors
Volume2
Issue number1
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'Molecular cloning, sequence analysis and mRNA expression of human ADP-ribosylation factor'. Together they form a unique fingerprint.

Cite this