Molecular cloning of the rat proteinase-activated receptor 4 (PAR4)

Willemijntje A. Hoogerwerf, Helen Lee Hellmich, Maria Adelaide Micci, John H. Winston, Lei Zhou, Pankaj J. Pasricha

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The proteinase-activated receptor 4 (PAR4) is a G-protein-coupled receptor activated by proteases such as thrombin and trypsin. Although activation of PAR4 has been shown to modulate rat gastrointestinal motility, the rat PAR4 sequence was unknown until now. This study aimed to identify the rat PAR4 cDNA. Results: The cDNA coding for the rat PAR4 homologue was cloned from the duodenum. Northern blots demonstrated a 3.0 kb transcript in the duodenum. Protein homology with mouse and human counterparts was 90% and 75% respectively. PAR4 is expressed predominantly in the esophagus, stomach, duodenum and the spleen. When expressed in COS cells, PAR4 is activated by trypsin (1 nM), thrombin (50 nM), mouse PAR4 specific peptide (500 μM) and a putative rat PAR4 specific activating peptide (100 μM), as measured by intracellular Ca2+-changes. Conclusions: We have identified and characterized cDNA encoding the rat PAR4 homologue. PAR4 is expressed predominantly in the upper gastrointestinal tract. It is activated by trypsin, thrombin and its newly identified rat PAR4 specific activating peptide.

Original languageEnglish (US)
Article number2
JournalBMC Molecular Biology
Volume3
DOIs
StatePublished - Feb 18 2002

ASJC Scopus subject areas

  • Molecular Biology

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