Molecular cloning of the human methylthioadenosine phosphorylase processed pseudogene and localization to 3q28

Phuoc T. Tran, Hiroki Hori, Yasuko Hori, Katsuzumi Okumura, Kazuhiro Kagotani, Hiroshi Taguchi, Dennis A. Carson, Tsutomu Nobori

Research output: Contribution to journalArticlepeer-review

Abstract

Human methylthioadenosine phosphorylase (MTAP) is a purine and methionine metabolic enzyme present ubiquitously in all normal tissues, but often deleted in many types of cancer. The gene for this enzyme maps to chromosome 9 at band p21 where the cyclin-dependent kinase inhibitor genes for p16 and p15 also reside. During our efforts to clone this gene we also isolated a phage clone containing a processed pseudogene of MTAP. The sequence is 92% homologous to the MTAP cDNA, is flanked at its 3' end by a repetitive element, but does not possess a poly(A) stretch. We localized this processed pseudogene to band 28 on the long arm of chromosome 3 by fluorescence in situ hybridization. All 22 malignant cell lines with deletions at 9p21 screened possessed the pseudogene.

Original languageEnglish (US)
Pages (from-to)263-269
Number of pages7
JournalGene
Volume186
Issue number2
DOIs
StatePublished - Feb 28 1997
Externally publishedYes

Keywords

  • Chemotherapy
  • False positive
  • Fluorescence in situ hybridization
  • Reference marker

ASJC Scopus subject areas

  • Genetics

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