Molecular cloning of the human methylthioadenosine phosphorylase processed pseudogene and localization to 3q28

Phuoc T. Tran; Hiroki Hori; Yasuko Hori; Katsuzumi Okumura; Kazuhiro Kagotani; Hiroshi Taguchi; Dennis A. Carson; Tsutomu Nobori

doi:10.1016/S0378-1119(96)00718-4

Molecular cloning of the human methylthioadenosine phosphorylase processed pseudogene and localization to 3q28

Phuoc T. Tran, Hiroki Hori, Yasuko Hori, Katsuzumi Okumura, Kazuhiro Kagotani, Hiroshi Taguchi, Dennis A. Carson, Tsutomu Nobori

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Human methylthioadenosine phosphorylase (MTAP) is a purine and methionine metabolic enzyme present ubiquitously in all normal tissues, but often deleted in many types of cancer. The gene for this enzyme maps to chromosome 9 at band p21 where the cyclin-dependent kinase inhibitor genes for p16 and p15 also reside. During our efforts to clone this gene we also isolated a phage clone containing a processed pseudogene of MTAP. The sequence is 92% homologous to the MTAP cDNA, is flanked at its 3' end by a repetitive element, but does not possess a poly(A) stretch. We localized this processed pseudogene to band 28 on the long arm of chromosome 3 by fluorescence in situ hybridization. All 22 malignant cell lines with deletions at 9p21 screened possessed the pseudogene.

Original language	English (US)
Pages (from-to)	263-269
Number of pages	7
Journal	Gene
Volume	186
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1119(96)00718-4
State	Published - Feb 28 1997
Externally published	Yes

Keywords

Chemotherapy
False positive
Fluorescence in situ hybridization
Reference marker

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(96)00718-4

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title = "Molecular cloning of the human methylthioadenosine phosphorylase processed pseudogene and localization to 3q28",

abstract = "Human methylthioadenosine phosphorylase (MTAP) is a purine and methionine metabolic enzyme present ubiquitously in all normal tissues, but often deleted in many types of cancer. The gene for this enzyme maps to chromosome 9 at band p21 where the cyclin-dependent kinase inhibitor genes for p16 and p15 also reside. During our efforts to clone this gene we also isolated a phage clone containing a processed pseudogene of MTAP. The sequence is 92% homologous to the MTAP cDNA, is flanked at its 3' end by a repetitive element, but does not possess a poly(A) stretch. We localized this processed pseudogene to band 28 on the long arm of chromosome 3 by fluorescence in situ hybridization. All 22 malignant cell lines with deletions at 9p21 screened possessed the pseudogene.",

keywords = "Chemotherapy, False positive, Fluorescence in situ hybridization, Reference marker",

author = "Tran, {Phuoc T.} and Hiroki Hori and Yasuko Hori and Katsuzumi Okumura and Kazuhiro Kagotani and Hiroshi Taguchi and Carson, {Dennis A.} and Tsutomu Nobori",

note = "Funding Information: We would like to thank L. Orvis and D. Wu for their excellent technical assistance. This work was supported in part by grants from the American Cancer Society (DHP-84), NIH (CA 64976) and a Grant-in-Aid for Creative Basic Research (Human Genome Program) from The Ministry of Education, Science and Culture of Japan.",

year = "1997",

month = feb,

day = "28",

doi = "10.1016/S0378-1119(96)00718-4",

language = "English (US)",

volume = "186",

pages = "263--269",

journal = "Gene",

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T1 - Molecular cloning of the human methylthioadenosine phosphorylase processed pseudogene and localization to 3q28

AU - Tran, Phuoc T.

AU - Hori, Hiroki

AU - Hori, Yasuko

AU - Okumura, Katsuzumi

AU - Kagotani, Kazuhiro

AU - Taguchi, Hiroshi

AU - Carson, Dennis A.

AU - Nobori, Tsutomu

N1 - Funding Information: We would like to thank L. Orvis and D. Wu for their excellent technical assistance. This work was supported in part by grants from the American Cancer Society (DHP-84), NIH (CA 64976) and a Grant-in-Aid for Creative Basic Research (Human Genome Program) from The Ministry of Education, Science and Culture of Japan.

PY - 1997/2/28

Y1 - 1997/2/28

N2 - Human methylthioadenosine phosphorylase (MTAP) is a purine and methionine metabolic enzyme present ubiquitously in all normal tissues, but often deleted in many types of cancer. The gene for this enzyme maps to chromosome 9 at band p21 where the cyclin-dependent kinase inhibitor genes for p16 and p15 also reside. During our efforts to clone this gene we also isolated a phage clone containing a processed pseudogene of MTAP. The sequence is 92% homologous to the MTAP cDNA, is flanked at its 3' end by a repetitive element, but does not possess a poly(A) stretch. We localized this processed pseudogene to band 28 on the long arm of chromosome 3 by fluorescence in situ hybridization. All 22 malignant cell lines with deletions at 9p21 screened possessed the pseudogene.

AB - Human methylthioadenosine phosphorylase (MTAP) is a purine and methionine metabolic enzyme present ubiquitously in all normal tissues, but often deleted in many types of cancer. The gene for this enzyme maps to chromosome 9 at band p21 where the cyclin-dependent kinase inhibitor genes for p16 and p15 also reside. During our efforts to clone this gene we also isolated a phage clone containing a processed pseudogene of MTAP. The sequence is 92% homologous to the MTAP cDNA, is flanked at its 3' end by a repetitive element, but does not possess a poly(A) stretch. We localized this processed pseudogene to band 28 on the long arm of chromosome 3 by fluorescence in situ hybridization. All 22 malignant cell lines with deletions at 9p21 screened possessed the pseudogene.

KW - Chemotherapy

KW - False positive

KW - Fluorescence in situ hybridization

KW - Reference marker

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AN - SCOPUS:0031035095

SN - 0378-1119

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JO - Gene

JF - Gene

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Molecular cloning of the human methylthioadenosine phosphorylase processed pseudogene and localization to 3q28

Abstract

Keywords

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