Molecular cloning of prepro-thyrotropin-releasing hormone cDNA from medaka (Oryzias latipes)

Yasuhiro Aoki, Tomohiro Masuda, Masayuki Iigo, Tadashi Yanagisawa

Research output: Contribution to journalArticlepeer-review

Abstract

The cDNA encoding prepro-thyrotropin-releasing hormone (ppTRH) in a teleost, medaka (Oryzias latipes) was isolated and characterized. The medaka ppTRH cDNA codes for 270 amino acid residues including eight TRH progenitor sequences (-Lys/Arg-Arg-Gln-His-Pro-Gly-Lys/Arg-Arg-). In silico analyses of the medaka genome database predicted that the structure of the medaka ppTRH gene is similar to the ppTRH genes of the other vertebrate species studied to date; consisting of three exons and two introns. Identity of the medaka ppTRH with the other vertebrates is rather low except the sockeye salmon. A molecular phylogenic tree showed that the ppTRH sequences reflected the predicted pattern of species classification. RT-PCR analysis demonstrated ppTRH gene expression in the brain and retina. These results gave some insight into the molecular evolution of ppTRH and physiological functions of TRH in vertebrates.

Original languageEnglish (US)
Pages (from-to)364-370
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume150
Issue number2
DOIs
StatePublished - Jan 15 2007

Keywords

  • Medaka Oryzias latipes
  • Molecular evolution
  • Prepro-thyrotropin-releasing hormone
  • Teleost
  • Thyrotropin-releasing hormone

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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