Molecular cloning of human Hic-5, a potential regulator involved in signal transduction and cellular senescence

Ji Zhang, Li Xin Zhang, Paul S. Meltzer, J. Carl Barrett, Jeffrey M. Trent

Research output: Contribution to journalArticle

Abstract

By using differential display, we cloned the human counterpart of the murine gene Hic-5 from senescent human keratinocytes. The full-length cDNA contained a short GC-stretch proceeding a consensus Kozak sequence followed by a single open reading frame of 1338 bp encoding a 461-amino acid protein with a predicted molecular weight of 50 kDa. The expression of this gene was prominent in cells of epithelial origin but low or absent in lymphoid tissues and hematopoietic cells. The deduced protein contained four LIM domains at the carboxyl-terminal end and four LD motifs at the amino-terminal half, sharing high similarities with the focal adhesion protein paxillin. Hic-5 may therefore function, like paxillin, as a potential adapter for the recruitment of structural and signaling molecules to certain subcellular sites or in focal adhesions. Isolation of the genomic sequence revealed that the gene covered a segment of 6 kb and spanned 11 exons from the translation initiation site ATG to the termination signal TGA. Fluorescent in situ hybridization by using a human Hic-5 specific probe localized the gene to human chromosome 16p11. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalMolecular Carcinogenesis
Volume27
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Cell Aging
Molecular Cloning
Paxillin
Signal Transduction
Focal Adhesions
Genes
Proteins
Consensus Sequence
Human Chromosomes
Lymphoid Tissue
Fluorescence In Situ Hybridization
Keratinocytes
Open Reading Frames
Exons
Complementary DNA
Molecular Weight
Epithelial Cells
Gene Expression
Amino Acids

Keywords

  • 16p11
  • Focal adhesion
  • Hic-5
  • p50
  • Senescence

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Molecular cloning of human Hic-5, a potential regulator involved in signal transduction and cellular senescence. / Zhang, Ji; Zhang, Li Xin; Meltzer, Paul S.; Barrett, J. Carl; Trent, Jeffrey M.

In: Molecular Carcinogenesis, Vol. 27, No. 3, 2000, p. 177-183.

Research output: Contribution to journalArticle

Zhang, Ji ; Zhang, Li Xin ; Meltzer, Paul S. ; Barrett, J. Carl ; Trent, Jeffrey M. / Molecular cloning of human Hic-5, a potential regulator involved in signal transduction and cellular senescence. In: Molecular Carcinogenesis. 2000 ; Vol. 27, No. 3. pp. 177-183.
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