Molecular cloning of human genes for serum amyloid A

Research output: Contribution to journalArticle

Abstract

Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalGene
Volume21
Issue number1-2
DOIs
StatePublished - Jan 1 1983

Keywords

  • Recombinant DNA
  • acute phase response
  • amyloidosis
  • plaque hybridization

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Molecular cloning of human genes for serum amyloid A'. Together they form a unique fingerprint.

  • Cite this