Molecular cloning of human genes for serum amyloid A

Research output: Contribution to journalArticle

Abstract

Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalGene
Volume21
Issue number1-2
DOIs
StatePublished - 1983

Fingerprint

Serum Amyloid A Protein
Molecular Cloning
Pluto
Acute-Phase Proteins
Amyloid
Bacteriophages
Genes
Plasmids
Complementary DNA
DNA
Enzymes

Keywords

  • acute phase response
  • amyloidosis
  • plaque hybridization
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Molecular cloning of human genes for serum amyloid A. / Sack, George Henry.

In: Gene, Vol. 21, No. 1-2, 1983, p. 19-24.

Research output: Contribution to journalArticle

@article{63a652823f054863afab797ed9573554,
title = "Molecular cloning of human genes for serum amyloid A",
abstract = "Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.",
keywords = "acute phase response, amyloidosis, plaque hybridization, Recombinant DNA",
author = "Sack, {George Henry}",
year = "1983",
doi = "10.1016/0378-1119(83)90143-9",
language = "English (US)",
volume = "21",
pages = "19--24",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Molecular cloning of human genes for serum amyloid A

AU - Sack, George Henry

PY - 1983

Y1 - 1983

N2 - Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.

AB - Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.

KW - acute phase response

KW - amyloidosis

KW - plaque hybridization

KW - Recombinant DNA

UR - http://www.scopus.com/inward/record.url?scp=0020548931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020548931&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(83)90143-9

DO - 10.1016/0378-1119(83)90143-9

M3 - Article

C2 - 6301947

AN - SCOPUS:0020548931

VL - 21

SP - 19

EP - 24

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -