TY - JOUR
T1 - Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium.
AU - Jones, D. T.
AU - Reed, R. R.
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 1987/10/15
Y1 - 1987/10/15
N2 - Biochemical studies in vertebrate olfactory tissue indicate that certain odorants stimulate adenylyl cyclase in a GTP-dependent manner. Additionally, immunochemical and toxin-labeling studies demonstrate the presence of several GTP-binding protein (G-protein) species in vertebrate olfactory epithelium. To identify the G-protein(s) responsible for olfactory signal transduction, we screened a rat olfactory cDNA library with an oligonucleotide probe and isolated 32 recombinant clones encoding five distinct types of G-protein alpha subunits. The majority of the clones encoded G alpha s, while the remaining clones encoded G alpha o, G alpha i1, G alpha i2, and a novel species, G alpha i3. Messenger RNA corresponding to each G alpha was detectable in all tissues examined; however, the levels for a given G alpha varied in a tissue-specific manner. In olfactory tissue, G alpha s was the most abundant of these messages and in combination with the biochemical studies suggests that G alpha s is the G-protein component of the olfactory signal transduction cascade.
AB - Biochemical studies in vertebrate olfactory tissue indicate that certain odorants stimulate adenylyl cyclase in a GTP-dependent manner. Additionally, immunochemical and toxin-labeling studies demonstrate the presence of several GTP-binding protein (G-protein) species in vertebrate olfactory epithelium. To identify the G-protein(s) responsible for olfactory signal transduction, we screened a rat olfactory cDNA library with an oligonucleotide probe and isolated 32 recombinant clones encoding five distinct types of G-protein alpha subunits. The majority of the clones encoded G alpha s, while the remaining clones encoded G alpha o, G alpha i1, G alpha i2, and a novel species, G alpha i3. Messenger RNA corresponding to each G alpha was detectable in all tissues examined; however, the levels for a given G alpha varied in a tissue-specific manner. In olfactory tissue, G alpha s was the most abundant of these messages and in combination with the biochemical studies suggests that G alpha s is the G-protein component of the olfactory signal transduction cascade.
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M3 - Article
C2 - 2820999
AN - SCOPUS:0023656556
VL - 262
SP - 14241
EP - 14249
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 29
ER -