Molecular cloning of Chinese hamster metallothionein II gene and its 5' flanking region

S. H. Hung, C. W. Yu, T. M. Chou, P C Huang, L. Y. Lin

Research output: Contribution to journalArticle

Abstract

A genomic DNA clone containing Chinese hamster metallothionein II (MTII) gene and its 5' flanking region was isolated from Cd resistant Chinese hamster ovary (CHO) cells. DNA sequence analysis showed that there are three exons and two introns in the structure of the MTII gene. Further characterization of the 5' flanking region reveals the possible transcription initiation site, metal responsive element and basal-level enhancer sequence. Putatively, this is the promoter region of CHO MTII gene.

Original languageEnglish (US)
Pages (from-to)255-258
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1090
Issue number2
StatePublished - 1991
Externally publishedYes

Fingerprint

Metallothionein
5' Flanking Region
Cloning
Molecular Cloning
Cricetulus
Genes
Ovary
Transcription Initiation Site
DNA sequences
DNA Sequence Analysis
Genetic Promoter Regions
Introns
Exons
Clone Cells
Metals
Cells
DNA

Keywords

  • Chinese hamster ovary cell
  • Genomic structure
  • Metallothionein II
  • Promoter sequence

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Molecular cloning of Chinese hamster metallothionein II gene and its 5' flanking region. / Hung, S. H.; Yu, C. W.; Chou, T. M.; Huang, P C; Lin, L. Y.

In: BBA - Gene Structure and Expression, Vol. 1090, No. 2, 1991, p. 255-258.

Research output: Contribution to journalArticle

Hung, S. H. ; Yu, C. W. ; Chou, T. M. ; Huang, P C ; Lin, L. Y. / Molecular cloning of Chinese hamster metallothionein II gene and its 5' flanking region. In: BBA - Gene Structure and Expression. 1991 ; Vol. 1090, No. 2. pp. 255-258.
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