Abstract
A genomic DNA clone containing Chinese hamster metallothionein II (MTII) gene and its 5' flanking region was isolated from Cd resistant Chinese hamster ovary (CHO) cells. DNA sequence analysis showed that there are three exons and two introns in the structure of the MTII gene. Further characterization of the 5' flanking region reveals the possible transcription initiation site, metal responsive element and basal-level enhancer sequence. Putatively, this is the promoter region of CHO MTII gene.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 255-258 |
| Number of pages | 4 |
| Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Volume | 1090 |
| Issue number | 2 |
| State | Published - Oct 23 1991 |
| Externally published | Yes |
Keywords
- Chinese hamster ovary cell
- Genomic structure
- Metallothionein II
- Promoter sequence
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics