Molecular cloning, molecular evolution and gene expression of cDNAs encoding thyrotropin-releasing hormone receptor subtypes in a teleost, the sockeye salmon (Oncorhynchus nerka)

Yuichi Saito, Miyuki Mekuchi, Noriaki Kobayashi, Makoto Kimura, Yasuhiro Aoki, Tomohiro Masuda, Teruo Azuma, Motohiro Fukami, Masayuki Iigo, Tadashi Yanagisawa

Research output: Contribution to journalArticle

Abstract

Molecular cloning of thyrotropin-releasing hormone receptors (TRHR) was performed in a teleost, the sockeye salmon (Oncorhynchus nerka). Four different TRHR cDNAs were cloned and named TRHR1, TRHR2a, TRHR2b and TRHR3 based on their similarity to known TRHR subtypes in vertebrates. Important residues for TRH binding were conserved in deduced amino acid sequences of the three TRHR subtypes except for the TRHR2b. Seven transmembrane domains were predicted for TRHR1, TRHR2a and TRHR3 proteins but only five for TRHR2b which appears to be truncated. In silico database analysis identified putative TRHR sequences including invertebrate TRHR and reptilian, avian and mammalian TRHR3. Phylogenetic analyses predicted the molecular evolution of TRHR in vertebrates: from the common ancestral TRHR (i.e. invertebrate TRHR), the TRHR2 subtype diverged first and then TRHR1 and TRHR3 diverged. Reverse transcription-polymerase chain reaction analyses revealed TRHR1 transcripts in the brain (hypothalamus), retina, pituitary gland and large intestine; TRHR2a in the brain (telencephalon and hypothalamus); and TRHR3 in the brain (olfactory bulbs) and retina.

Original languageEnglish (US)
Pages (from-to)80-88
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume174
Issue number2
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

Fingerprint

Thyrotropin Releasing Hormone Receptors
thyrotropin-releasing hormone
Oncorhynchus nerka
Molecular Evolution
Salmon
hormone receptors
Molecular Cloning
molecular cloning
Complementary DNA
Gene Expression
gene expression
Invertebrate Hormones
hypothalamus
brain
retina
Hypothalamus
Vertebrates
Retina
Brain
invertebrates

Keywords

  • Molecular evolution
  • Sockeye salmon Oncorhynchus nerka
  • Teleosts
  • Thyrotropin-releasing hormone
  • Thyrotropin-releasing hormone receptor

ASJC Scopus subject areas

  • Endocrinology

Cite this

Molecular cloning, molecular evolution and gene expression of cDNAs encoding thyrotropin-releasing hormone receptor subtypes in a teleost, the sockeye salmon (Oncorhynchus nerka). / Saito, Yuichi; Mekuchi, Miyuki; Kobayashi, Noriaki; Kimura, Makoto; Aoki, Yasuhiro; Masuda, Tomohiro; Azuma, Teruo; Fukami, Motohiro; Iigo, Masayuki; Yanagisawa, Tadashi.

In: General and Comparative Endocrinology, Vol. 174, No. 2, 01.11.2011, p. 80-88.

Research output: Contribution to journalArticle

Saito, Yuichi ; Mekuchi, Miyuki ; Kobayashi, Noriaki ; Kimura, Makoto ; Aoki, Yasuhiro ; Masuda, Tomohiro ; Azuma, Teruo ; Fukami, Motohiro ; Iigo, Masayuki ; Yanagisawa, Tadashi. / Molecular cloning, molecular evolution and gene expression of cDNAs encoding thyrotropin-releasing hormone receptor subtypes in a teleost, the sockeye salmon (Oncorhynchus nerka). In: General and Comparative Endocrinology. 2011 ; Vol. 174, No. 2. pp. 80-88.
@article{5c8c465d52c04fa2a9083d155a033c8a,
title = "Molecular cloning, molecular evolution and gene expression of cDNAs encoding thyrotropin-releasing hormone receptor subtypes in a teleost, the sockeye salmon (Oncorhynchus nerka)",
abstract = "Molecular cloning of thyrotropin-releasing hormone receptors (TRHR) was performed in a teleost, the sockeye salmon (Oncorhynchus nerka). Four different TRHR cDNAs were cloned and named TRHR1, TRHR2a, TRHR2b and TRHR3 based on their similarity to known TRHR subtypes in vertebrates. Important residues for TRH binding were conserved in deduced amino acid sequences of the three TRHR subtypes except for the TRHR2b. Seven transmembrane domains were predicted for TRHR1, TRHR2a and TRHR3 proteins but only five for TRHR2b which appears to be truncated. In silico database analysis identified putative TRHR sequences including invertebrate TRHR and reptilian, avian and mammalian TRHR3. Phylogenetic analyses predicted the molecular evolution of TRHR in vertebrates: from the common ancestral TRHR (i.e. invertebrate TRHR), the TRHR2 subtype diverged first and then TRHR1 and TRHR3 diverged. Reverse transcription-polymerase chain reaction analyses revealed TRHR1 transcripts in the brain (hypothalamus), retina, pituitary gland and large intestine; TRHR2a in the brain (telencephalon and hypothalamus); and TRHR3 in the brain (olfactory bulbs) and retina.",
keywords = "Molecular evolution, Sockeye salmon Oncorhynchus nerka, Teleosts, Thyrotropin-releasing hormone, Thyrotropin-releasing hormone receptor",
author = "Yuichi Saito and Miyuki Mekuchi and Noriaki Kobayashi and Makoto Kimura and Yasuhiro Aoki and Tomohiro Masuda and Teruo Azuma and Motohiro Fukami and Masayuki Iigo and Tadashi Yanagisawa",
year = "2011",
month = "11",
day = "1",
doi = "10.1016/j.ygcen.2011.07.011",
language = "English (US)",
volume = "174",
pages = "80--88",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Molecular cloning, molecular evolution and gene expression of cDNAs encoding thyrotropin-releasing hormone receptor subtypes in a teleost, the sockeye salmon (Oncorhynchus nerka)

AU - Saito, Yuichi

AU - Mekuchi, Miyuki

AU - Kobayashi, Noriaki

AU - Kimura, Makoto

AU - Aoki, Yasuhiro

AU - Masuda, Tomohiro

AU - Azuma, Teruo

AU - Fukami, Motohiro

AU - Iigo, Masayuki

AU - Yanagisawa, Tadashi

PY - 2011/11/1

Y1 - 2011/11/1

N2 - Molecular cloning of thyrotropin-releasing hormone receptors (TRHR) was performed in a teleost, the sockeye salmon (Oncorhynchus nerka). Four different TRHR cDNAs were cloned and named TRHR1, TRHR2a, TRHR2b and TRHR3 based on their similarity to known TRHR subtypes in vertebrates. Important residues for TRH binding were conserved in deduced amino acid sequences of the three TRHR subtypes except for the TRHR2b. Seven transmembrane domains were predicted for TRHR1, TRHR2a and TRHR3 proteins but only five for TRHR2b which appears to be truncated. In silico database analysis identified putative TRHR sequences including invertebrate TRHR and reptilian, avian and mammalian TRHR3. Phylogenetic analyses predicted the molecular evolution of TRHR in vertebrates: from the common ancestral TRHR (i.e. invertebrate TRHR), the TRHR2 subtype diverged first and then TRHR1 and TRHR3 diverged. Reverse transcription-polymerase chain reaction analyses revealed TRHR1 transcripts in the brain (hypothalamus), retina, pituitary gland and large intestine; TRHR2a in the brain (telencephalon and hypothalamus); and TRHR3 in the brain (olfactory bulbs) and retina.

AB - Molecular cloning of thyrotropin-releasing hormone receptors (TRHR) was performed in a teleost, the sockeye salmon (Oncorhynchus nerka). Four different TRHR cDNAs were cloned and named TRHR1, TRHR2a, TRHR2b and TRHR3 based on their similarity to known TRHR subtypes in vertebrates. Important residues for TRH binding were conserved in deduced amino acid sequences of the three TRHR subtypes except for the TRHR2b. Seven transmembrane domains were predicted for TRHR1, TRHR2a and TRHR3 proteins but only five for TRHR2b which appears to be truncated. In silico database analysis identified putative TRHR sequences including invertebrate TRHR and reptilian, avian and mammalian TRHR3. Phylogenetic analyses predicted the molecular evolution of TRHR in vertebrates: from the common ancestral TRHR (i.e. invertebrate TRHR), the TRHR2 subtype diverged first and then TRHR1 and TRHR3 diverged. Reverse transcription-polymerase chain reaction analyses revealed TRHR1 transcripts in the brain (hypothalamus), retina, pituitary gland and large intestine; TRHR2a in the brain (telencephalon and hypothalamus); and TRHR3 in the brain (olfactory bulbs) and retina.

KW - Molecular evolution

KW - Sockeye salmon Oncorhynchus nerka

KW - Teleosts

KW - Thyrotropin-releasing hormone

KW - Thyrotropin-releasing hormone receptor

UR - http://www.scopus.com/inward/record.url?scp=80054095384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054095384&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2011.07.011

DO - 10.1016/j.ygcen.2011.07.011

M3 - Article

C2 - 21827760

AN - SCOPUS:80054095384

VL - 174

SP - 80

EP - 88

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 2

ER -