TY - JOUR
T1 - Molecular cloning and mapping of the human nicotinic acetylcholine receptor α10 (CHRNA10)
AU - Lustig, Lawrence R.
AU - Peng, Huashan
AU - Hiel, Hakim
AU - Yamamoto, Takehito
AU - Fuchs, Paul A.
PY - 2001/5/1
Y1 - 2001/5/1
N2 - We report the isolation and initial characterization of a new member of the human nicotinic acetylcholine receptor (nAChR) subunit family, α10 (CHRNA10), from both inner-ear neuroepithelium and lymphoid tissue. The cDNA is 1959 nucleotides in length, with a coding region predicting a protein of 451 amino acids that is 90% identical to rat α10. The α10 gene was localized to chromosome 11p15.5. Human α10 was detected in human inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes using reverse transcriptase-polymerase chain reaction, Northern blot hybridization, and immunohistochemistry. We also detected the expression of the human nAChR α9 (CHRNA9) mRNA in these same tissues using RT-PCR and Northern blot hybridization.
AB - We report the isolation and initial characterization of a new member of the human nicotinic acetylcholine receptor (nAChR) subunit family, α10 (CHRNA10), from both inner-ear neuroepithelium and lymphoid tissue. The cDNA is 1959 nucleotides in length, with a coding region predicting a protein of 451 amino acids that is 90% identical to rat α10. The α10 gene was localized to chromosome 11p15.5. Human α10 was detected in human inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes using reverse transcriptase-polymerase chain reaction, Northern blot hybridization, and immunohistochemistry. We also detected the expression of the human nAChR α9 (CHRNA9) mRNA in these same tissues using RT-PCR and Northern blot hybridization.
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U2 - 10.1006/geno.2000.6503
DO - 10.1006/geno.2000.6503
M3 - Article
C2 - 11350119
AN - SCOPUS:0035338575
SN - 0888-7543
VL - 73
SP - 272
EP - 283
JO - Genomics
JF - Genomics
IS - 3
ER -