Molecular cloning and mapping of the human nicotinic acetylcholine receptor α10 (CHRNA10)

Lawrence R. Lustig, Huashan Peng, Hakim Hiel, Takehito Yamamoto, Paul A. Fuchs

Research output: Contribution to journalArticlepeer-review

Abstract

We report the isolation and initial characterization of a new member of the human nicotinic acetylcholine receptor (nAChR) subunit family, α10 (CHRNA10), from both inner-ear neuroepithelium and lymphoid tissue. The cDNA is 1959 nucleotides in length, with a coding region predicting a protein of 451 amino acids that is 90% identical to rat α10. The α10 gene was localized to chromosome 11p15.5. Human α10 was detected in human inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes using reverse transcriptase-polymerase chain reaction, Northern blot hybridization, and immunohistochemistry. We also detected the expression of the human nAChR α9 (CHRNA9) mRNA in these same tissues using RT-PCR and Northern blot hybridization.

Original languageEnglish (US)
Pages (from-to)272-283
Number of pages12
JournalGenomics
Volume73
Issue number3
DOIs
StatePublished - May 1 2001

ASJC Scopus subject areas

  • Genetics

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