Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney

Andrea Rasola, Luis J V Galietta, Virginia Barone, Giovanni Romeo, Serena M Bagnasco

Research output: Contribution to journalArticle

Abstract

The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a Km = 20 μM for GABA transport and a coupling to Na+ and Cl- with a stoichiometry 3 Na+:2 Cl-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalFEBS Letters
Volume373
Issue number3
DOIs
StatePublished - Oct 16 1995
Externally publishedYes

Fingerprint

GABA Plasma Membrane Transport Proteins
Cloning
Molecular Cloning
gamma-Aminobutyric Acid
Kidney
Neurotransmitter Transport Proteins
Phloretin
Kidney Medulla
Betaine
Quinidine
Verapamil
Gene Library
Placenta
Canidae
Myocardium
Skeletal Muscle
Chromosomes
Stoichiometry
Liver
Muscle

Keywords

  • Betaine
  • Cell volume
  • Chromosomal localization
  • GABA
  • Kidney
  • Transporter

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney. / Rasola, Andrea; Galietta, Luis J V; Barone, Virginia; Romeo, Giovanni; Bagnasco, Serena M.

In: FEBS Letters, Vol. 373, No. 3, 16.10.1995, p. 229-233.

Research output: Contribution to journalArticle

Rasola, Andrea ; Galietta, Luis J V ; Barone, Virginia ; Romeo, Giovanni ; Bagnasco, Serena M. / Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney. In: FEBS Letters. 1995 ; Vol. 373, No. 3. pp. 229-233.
@article{2bb20a7ef0ba4421a6a099f56aaf6750,
title = "Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney",
abstract = "The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a Km = 20 μM for GABA transport and a coupling to Na+ and Cl- with a stoichiometry 3 Na+:2 Cl-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.",
keywords = "Betaine, Cell volume, Chromosomal localization, GABA, Kidney, Transporter",
author = "Andrea Rasola and Galietta, {Luis J V} and Virginia Barone and Giovanni Romeo and Bagnasco, {Serena M}",
year = "1995",
month = "10",
day = "16",
doi = "10.1016/0014-5793(95)01052-G",
language = "English (US)",
volume = "373",
pages = "229--233",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney

AU - Rasola, Andrea

AU - Galietta, Luis J V

AU - Barone, Virginia

AU - Romeo, Giovanni

AU - Bagnasco, Serena M

PY - 1995/10/16

Y1 - 1995/10/16

N2 - The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a Km = 20 μM for GABA transport and a coupling to Na+ and Cl- with a stoichiometry 3 Na+:2 Cl-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.

AB - The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a Km = 20 μM for GABA transport and a coupling to Na+ and Cl- with a stoichiometry 3 Na+:2 Cl-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.

KW - Betaine

KW - Cell volume

KW - Chromosomal localization

KW - GABA

KW - Kidney

KW - Transporter

UR - http://www.scopus.com/inward/record.url?scp=0028786744&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028786744&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(95)01052-G

DO - 10.1016/0014-5793(95)01052-G

M3 - Article

C2 - 7589472

AN - SCOPUS:0028786744

VL - 373

SP - 229

EP - 233

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -