Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney

Andrea Rasola, Luis J.V. Galietta, Virginia Barone, Giovanni Romeo, Serena Bagnasco

Research output: Contribution to journalArticle

Abstract

The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a Km = 20 μM for GABA transport and a coupling to Na+ and Cl- with a stoichiometry 3 Na+:2 Cl-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalFEBS Letters
Volume373
Issue number3
DOIs
StatePublished - Oct 16 1995
Externally publishedYes

Keywords

  • Betaine
  • Cell volume
  • Chromosomal localization
  • GABA
  • Kidney
  • Transporter

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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