TY - JOUR
T1 - Molecular cloning and characterization of the human budding uninhibited by benomyl (BUB3) promoter
AU - Baek, Won Ki
AU - Park, Jong Wook
AU - Lim, Jun Hee
AU - Suh, Seong Il
AU - Suh, Min Ho
AU - Gabrielson, Edward
AU - Kwon, Taeg Kyu
N1 - Funding Information:
This work was supported by a research-promoting grant from the Keimyung University Dongsan Medical Center in 2000.
PY - 2002/7/24
Y1 - 2002/7/24
N2 - Recently, cDNA corresponding to the human homologue of the BUB3 (budding uninhibited by benomyl) mitotic checkpoint protein has been identified and cloned. Previous studies from our laboratory and others have found this gene to localize to 10q26, a region that is frequently altered in various human cancers. We describe here a series of studies designed to understand the genomic structure of BUB3, particularly as it relates to regulation of gene expression. The human BUB3 gene has seven exons and six introns, and spans a genomic region of over 16 kb. The four WD repeat sequences in this gene are localized to exons 2, 4, and 6, and there is a major transcriptional start site located 554 nucleotides upstream of the ATG translation initiator codon. The promoter region lacks a TATA box but contains potential binding sites for the transcriptional factors including SP1, E2F, c-Myc, C/EBP and NFκB. To analyse the regulatory mechanisms controlling hBUB3 gene expression, we characterized the 5′-flanking region from nucleotide -1.3 to +0.58 kb by cloning various potions of this region in front of a luciferase reporter sequence. These experiments indicate that this region 5′ region contains distinctive positive and negative regulatory elements.
AB - Recently, cDNA corresponding to the human homologue of the BUB3 (budding uninhibited by benomyl) mitotic checkpoint protein has been identified and cloned. Previous studies from our laboratory and others have found this gene to localize to 10q26, a region that is frequently altered in various human cancers. We describe here a series of studies designed to understand the genomic structure of BUB3, particularly as it relates to regulation of gene expression. The human BUB3 gene has seven exons and six introns, and spans a genomic region of over 16 kb. The four WD repeat sequences in this gene are localized to exons 2, 4, and 6, and there is a major transcriptional start site located 554 nucleotides upstream of the ATG translation initiator codon. The promoter region lacks a TATA box but contains potential binding sites for the transcriptional factors including SP1, E2F, c-Myc, C/EBP and NFκB. To analyse the regulatory mechanisms controlling hBUB3 gene expression, we characterized the 5′-flanking region from nucleotide -1.3 to +0.58 kb by cloning various potions of this region in front of a luciferase reporter sequence. These experiments indicate that this region 5′ region contains distinctive positive and negative regulatory elements.
KW - BUB3
KW - Mitotic checkpoint protein
KW - Promoter
KW - Transcription factor
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U2 - 10.1016/S0378-1119(02)00827-2
DO - 10.1016/S0378-1119(02)00827-2
M3 - Article
C2 - 12242018
AN - SCOPUS:0037167173
SN - 0378-1119
VL - 295
SP - 117
EP - 123
JO - Gene
JF - Gene
IS - 1
ER -