Molecular cloning and characterization of an aquaporin cDNA from salivary, lacrimal, and respiratory tissues

S. Raina, G. M. Preston, William B Guggino, Peter C Agre

Research output: Contribution to journalArticle

Abstract

The Aquaporin family of water channels plays a fundamental role in transmembrane water movements in numerous plant and animal tissues. Since the molecular pathway by which water is secreted by salivary glands is unknown, a cDNA was isolated from rat submandibular gland by homology cloning. Similar to other Aquaporins, the salivary cDNA encodes a 265-residue polypeptide with six putative transmembrane domains separated by five connecting loops (A-E); the NH2- and COOH-terminal halves of the polypeptide are sequence-related, and each contains the motif Asn-Pro-Ala. A mercurial-inhibition site is present in extracellular loop E, and cytoplasmic loop D contains a cAMP- protein kinase phosphorylation consensus. In vitro translation yielded a 27- kDa polypeptide, and expression of the cRNA in Xenopus oocytes conferred a 20-fold increase in osmotic water permeability (P(f)) which was reversibly inhibited by 1 mM HgCl2. Northern analysis demonstrated a 1.6-kilobase mRNA in submandibular, parotid, and sublingual salivary glands, lacrimal gland, eye, trachea, and lung. In situ hybridization revealed a strong hybridization over the corneal epithelium in eye and over the secretory lobules in salivary glands. These studies have identified a new mammalian member of the Aquaporin water channel family (gene symbol AQP5) which is implicated in the generation of saliva, tears, and pulmonary secretions.

Original languageEnglish (US)
Pages (from-to)1908-1912
Number of pages5
JournalJournal of Biological Chemistry
Volume270
Issue number4
DOIs
StatePublished - 1995

Fingerprint

Aquaporins
Cloning
Molecular Cloning
Tears
Complementary DNA
Tissue
Salivary Glands
Peptides
Water
Sublingual Gland
Water Movements
Lacrimal Apparatus
Complementary RNA
Lung
Mercuric Chloride
Corneal Epithelium
Phosphorylation
Submandibular Gland
Xenopus
Trachea

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and characterization of an aquaporin cDNA from salivary, lacrimal, and respiratory tissues. / Raina, S.; Preston, G. M.; Guggino, William B; Agre, Peter C.

In: Journal of Biological Chemistry, Vol. 270, No. 4, 1995, p. 1908-1912.

Research output: Contribution to journalArticle

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