Molecular cloning and analysis of the expression of the E1β subunit of branched chain α-ketoacid dehydrogenase in mice

Jeffrey M. Chinsky, Paul A. Costeas

Research output: Contribution to journalArticle

Abstract

The cDNA sequence encoding the murine liver E1β subunit of the branched-chain α-ketoacid dehydrogenase complex (BCKAD) was determined. In the region encoding the mature E1β subunit protein, both the nucleotide composition and predicted amino acid sequence are highly conserved between murine, rat, human, and bovine species. In contrast, they are less well conserved in the 5′ sequence encoding the amino terminal preprotein sequence and 3′ untranslated region. The pattern of tissue-specific expression of three BCKAD subunit RNAs was determined to be similar in both rat and murine tissues except for that observed in murine liver, where a higher than expected level of E1β subunit RNA was observed. Variation in the levels of this subunit might play a major role in the regulation of the overall ability of the murine liver to modulate BCKAD content in response to changing physiologic needs.

Original languageEnglish (US)
Pages (from-to)499-503
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1216
Issue number3
DOIs
StatePublished - Dec 14 1993
Externally publishedYes

Keywords

  • (Mouse liver)
  • Branched-chain α-ketoacid dehydrogenase
  • E1β subunit
  • Ketoacid dehydrogenase
  • Murine tissue-specific expression
  • RNA
  • cDNA

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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