@article{1c9b5ad1626444e19f054142b07ef407,
title = "Molecular cloning and analysis of the expression of the E1β subunit of branched chain α-ketoacid dehydrogenase in mice",
abstract = "The cDNA sequence encoding the murine liver E1β subunit of the branched-chain α-ketoacid dehydrogenase complex (BCKAD) was determined. In the region encoding the mature E1β subunit protein, both the nucleotide composition and predicted amino acid sequence are highly conserved between murine, rat, human, and bovine species. In contrast, they are less well conserved in the 5′ sequence encoding the amino terminal preprotein sequence and 3′ untranslated region. The pattern of tissue-specific expression of three BCKAD subunit RNAs was determined to be similar in both rat and murine tissues except for that observed in murine liver, where a higher than expected level of E1β subunit RNA was observed. Variation in the levels of this subunit might play a major role in the regulation of the overall ability of the murine liver to modulate BCKAD content in response to changing physiologic needs.",
keywords = "(Mouse liver), Branched-chain α-ketoacid dehydrogenase, E1β subunit, Ketoacid dehydrogenase, Murine tissue-specific expression, RNA, cDNA",
author = "Chinsky, {Jeffrey M.} and Costeas, {Paul A.}",
note = "Funding Information: nonhepatict issues in mice but was apparently dis-crepanti n murine liver samples. Overall, our studies suggestt hat there might be differencesin the control of expressiono f the individual BCKAD subunitsi n murine liver. The variability in the liver/kidneyr atios observedf or El/3 but not Ela may reflect a mechanismo f regulationo f murine hepatic BCKAD E1/3 subunit RNA expressiont hat may not affect Elc~. This would suggestt he possibilityt hat this type of regulationm ay representa point of control in the overalla bility of the liver to modulateit s content of functionally complete BCKAD complex. Experiments are underwayt o determineu nder which conditions expressiono f El/3 in the murine liver might vary to regulatet he overall level of BCKAD activity of the liver. Preliminary data suggestt hat levels of this subunit RNA in the liver variesw ith dietary protein composition( J. Chinsky, unpublishedd ata). The availability of the clonedm urine El/3 cDNA with more extensive 5' rodent sequencei,n cluding the entire coding sequencef or the preprotein, should aid attemptst o isolatet he genomicp romoterr egions equencen eeded to study the basis of such regulation. The excellentt echnicala ssistanceo f Lizabeth M. Bohlen was greatly appreciatedW. e thank Yu Zhao and Robert Harris, Departmento f Biochemistrya nd Molecular Biology, Indiana University School of Medicine, Indianapolis, IN for sharing their rat BCKAD E1/3 and E2 cDNA sequencews ith us prior to publicationa s well as the Ela cDNA \[10,14,15T\]h. e murine skeletalm usclecDNA library was generously suppliedb y the laboratory of C. Thomas Caskey, Bay-lor College of Medicine, Houston,T X. This work was supportedi n part by grants from the Life and Health Insurance Medical ResearchF und and the Frank C. BresslerR esearchF und of the Universityo f Maryland School of Medicine.",
year = "1993",
month = dec,
day = "14",
doi = "10.1016/0167-4781(93)90023-7",
language = "English (US)",
volume = "1216",
pages = "499--503",
journal = "BBA - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "3",
}