Molecular characterization of the Taenia solium Tso31 antigen and homologous of other Taenia species from Peru

for the Cysticercosis Working Group in Peru

Research output: Contribution to journalArticle

Abstract

Several studies have been performed to determine specific antigens for the diagnosis of tapeworms. One of these antigens is Tso31, which is used to differentiate Taenia solium and Taenia saginata in human feces. The aim of the present work was the molecular characterization of this protein in different tapeworm specimens collected in Peru: T. omisa (n = 6), T. hydatigena (n = 7), T. taeniaeformis (n = 4), T. pisiformes (n = 1), T. multiceps (n = 7), and T. solium (n = 10). Total DNA was extracted from each proglottid using a commercial DNA kit for tissue. A nested PCR was used to amplify a fragment of the previously described oncosphere-specific protein Tso31 gene. The nested PCR products were analyzed by 1.5% agarose gel electrophoresis and visualized after ethidium bromide staining. All nested PCR-positive products were sequenced and their sequences were compared. Of all the tapeworms analyzed, only T. solium and T. multiceps amplified the Tso31 gene. All sequences were identical for each species. Our T. solium Tso31 showed 100% similarity when compared with published GenBank sequences. The difference between T. solium and T. multiceps Tso31 samples was 8.1%. In conclusion, our results show that the tsol31 gene is not exclusive to T. solium.

Original languageEnglish (US)
JournalParasitology Research
DOIs
StateAccepted/In press - Jan 1 2019
Externally publishedYes

Fingerprint

Taenia solium
Taenia
Peru
antigens
Antigens
Cestoda
tapeworms
Polymerase Chain Reaction
Taenia saginata
oncospheres
Ethidium
genes
Agar Gel Electrophoresis
DNA
Nucleic Acid Databases
Feces
agarose
gel electrophoresis
Genes
Proteins

Keywords

  • Homologous
  • Taenia solium
  • Tapeworms
  • Tso31 gene

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Molecular characterization of the Taenia solium Tso31 antigen and homologous of other Taenia species from Peru. / for the Cysticercosis Working Group in Peru.

In: Parasitology Research, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "Several studies have been performed to determine specific antigens for the diagnosis of tapeworms. One of these antigens is Tso31, which is used to differentiate Taenia solium and Taenia saginata in human feces. The aim of the present work was the molecular characterization of this protein in different tapeworm specimens collected in Peru: T. omisa (n = 6), T. hydatigena (n = 7), T. taeniaeformis (n = 4), T. pisiformes (n = 1), T. multiceps (n = 7), and T. solium (n = 10). Total DNA was extracted from each proglottid using a commercial DNA kit for tissue. A nested PCR was used to amplify a fragment of the previously described oncosphere-specific protein Tso31 gene. The nested PCR products were analyzed by 1.5{\%} agarose gel electrophoresis and visualized after ethidium bromide staining. All nested PCR-positive products were sequenced and their sequences were compared. Of all the tapeworms analyzed, only T. solium and T. multiceps amplified the Tso31 gene. All sequences were identical for each species. Our T. solium Tso31 showed 100{\%} similarity when compared with published GenBank sequences. The difference between T. solium and T. multiceps Tso31 samples was 8.1{\%}. In conclusion, our results show that the tsol31 gene is not exclusive to T. solium.",
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