Molecular characterization of the DNA puff C-8 gene of Rhynchosciara americana

Horácio M. Frydman, Esteban Osorio Cadavid, Jonny Yokosawa, Flavio Henrique Silva, Lucy Deise Navarro-Cattapan, Roberto Vicente Santelli, Marcelo Jacobs-Lorena, Monika Graessmann, Adolf Graessmann, Ann Jacob Stocker, F. J.S. Lara

Research output: Contribution to journalArticle

Abstract

We have mapped the only transcription unit known to be present in the C-8 DNA puff of Rhynchosciara americana and describe the isolation and sequence of a cDNA clone, pRa C-8-22, which contains a nearly complete copy of the mRNA transcribed from this DNA puff and part of the sequence of genomic clone BSC8-0.9, which contains the promoter region and the remainder of the transcription unit. The characteristics of the protein predicted from the ORF present in the cDNA indicate that it is unique and secreted.

Original languageEnglish (US)
Pages (from-to)799-803
Number of pages5
JournalJournal of molecular biology
Volume233
Issue number4
DOIs
StatePublished - Dec 20 1993
Externally publishedYes

Keywords

  • C-8 cDNA
  • C-8 mrNA
  • DNA puff
  • Rhynchosciara
  • Salivary glands

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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  • Cite this

    Frydman, H. M., Cadavid, E. O., Yokosawa, J., Silva, F. H., Navarro-Cattapan, L. D., Santelli, R. V., Jacobs-Lorena, M., Graessmann, M., Graessmann, A., Stocker, A. J., & Lara, F. J. S. (1993). Molecular characterization of the DNA puff C-8 gene of Rhynchosciara americana. Journal of molecular biology, 233(4), 799-803. https://doi.org/10.1006/jmbi.1993.1556