Molecular characteristics of Hepatitis B and chronic liver disease in a cohort of HB carriers from Bamako, Mali

Fatou Traoré, Emmanuelle Gormally, Stéphanie Villar, Marlin D. Friesen, John Davis Groopman, Guy Vernet, Souleymane Diallo, Pierre Hainaut, Moussa Y. Maiga

Research output: Contribution to journalArticle

Abstract

Background: Hepatitis B (HB) infection is common in Mali. However, there is little information on molecular and biochemical characteristics of HB carriers. Methods: A group of 1466 adult volunteers was recruited in the district of Bamako. Confirmed HB carriers were tested for HB viral load by quantitative PCR and HBV was genotyped by sequencing of HBS. Fibrosis and hepatitis activity were measured using the Fibrotest-Actitest. A mutation of TP53 at codon 249 (R249S), specific for exposure to aflatoxin, was detected in cell-free DNA extracted from plasma. Results: Overall, 276 subjects were HBsAg-positive (18.8%). Among 152 subjects tested for HBV load, 49 (32.2%) had over 104 copies/mL and 16 (10.5%) had levels below the limit of detection. The E genotype was found in 91.1% of carriers. Fibrotest scores ≥ F2 were observed in 52 subjects (35.4%). Actitest scores ≥ A2 were detected in 15 subjects (10.2%) and were correlated with Fibrotest scores (p = 0.0006). Among 105 subjects tested, 60% had detectable levels of R249S copies (>40 copies/mL plasma). Conclusion: Chronic HB carriage in adults in Bamako district is well over epidemic threshold. About 1/3 of carriers have moderate to severe liver fibrosis and 60% have detectable aflatoxin-related TP53 R249S mutation. These results support introduction of anti-HB therapies to reduce the progression towards severe liver disease.

Original languageEnglish (US)
Article number180
JournalBMC Infectious Diseases
Volume15
Issue number1
DOIs
StatePublished - Apr 11 2015

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Mali
Hepatitis B
Liver Diseases
Chronic Disease
Aflatoxins
Mutation
Chronic Hepatitis B
Hepatitis B Surface Antigens
Viral Load
varespladib methyl
Codon
Liver Cirrhosis
Hepatitis
Limit of Detection
Volunteers
Fibrosis
Genotype
Polymerase Chain Reaction
DNA
Infection

Keywords

  • Chronic carriage
  • Fibrotest/Actitest
  • HBV
  • Mali
  • TP53 R249S mutation
  • Viral load

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Molecular characteristics of Hepatitis B and chronic liver disease in a cohort of HB carriers from Bamako, Mali. / Traoré, Fatou; Gormally, Emmanuelle; Villar, Stéphanie; Friesen, Marlin D.; Groopman, John Davis; Vernet, Guy; Diallo, Souleymane; Hainaut, Pierre; Maiga, Moussa Y.

In: BMC Infectious Diseases, Vol. 15, No. 1, 180, 11.04.2015.

Research output: Contribution to journalArticle

Traoré, F, Gormally, E, Villar, S, Friesen, MD, Groopman, JD, Vernet, G, Diallo, S, Hainaut, P & Maiga, MY 2015, 'Molecular characteristics of Hepatitis B and chronic liver disease in a cohort of HB carriers from Bamako, Mali', BMC Infectious Diseases, vol. 15, no. 1, 180. https://doi.org/10.1186/s12879-015-0916-x
Traoré, Fatou ; Gormally, Emmanuelle ; Villar, Stéphanie ; Friesen, Marlin D. ; Groopman, John Davis ; Vernet, Guy ; Diallo, Souleymane ; Hainaut, Pierre ; Maiga, Moussa Y. / Molecular characteristics of Hepatitis B and chronic liver disease in a cohort of HB carriers from Bamako, Mali. In: BMC Infectious Diseases. 2015 ; Vol. 15, No. 1.
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AU - Gormally, Emmanuelle

AU - Villar, Stéphanie

AU - Friesen, Marlin D.

AU - Groopman, John Davis

AU - Vernet, Guy

AU - Diallo, Souleymane

AU - Hainaut, Pierre

AU - Maiga, Moussa Y.

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N2 - Background: Hepatitis B (HB) infection is common in Mali. However, there is little information on molecular and biochemical characteristics of HB carriers. Methods: A group of 1466 adult volunteers was recruited in the district of Bamako. Confirmed HB carriers were tested for HB viral load by quantitative PCR and HBV was genotyped by sequencing of HBS. Fibrosis and hepatitis activity were measured using the Fibrotest-Actitest. A mutation of TP53 at codon 249 (R249S), specific for exposure to aflatoxin, was detected in cell-free DNA extracted from plasma. Results: Overall, 276 subjects were HBsAg-positive (18.8%). Among 152 subjects tested for HBV load, 49 (32.2%) had over 104 copies/mL and 16 (10.5%) had levels below the limit of detection. The E genotype was found in 91.1% of carriers. Fibrotest scores ≥ F2 were observed in 52 subjects (35.4%). Actitest scores ≥ A2 were detected in 15 subjects (10.2%) and were correlated with Fibrotest scores (p = 0.0006). Among 105 subjects tested, 60% had detectable levels of R249S copies (>40 copies/mL plasma). Conclusion: Chronic HB carriage in adults in Bamako district is well over epidemic threshold. About 1/3 of carriers have moderate to severe liver fibrosis and 60% have detectable aflatoxin-related TP53 R249S mutation. These results support introduction of anti-HB therapies to reduce the progression towards severe liver disease.

AB - Background: Hepatitis B (HB) infection is common in Mali. However, there is little information on molecular and biochemical characteristics of HB carriers. Methods: A group of 1466 adult volunteers was recruited in the district of Bamako. Confirmed HB carriers were tested for HB viral load by quantitative PCR and HBV was genotyped by sequencing of HBS. Fibrosis and hepatitis activity were measured using the Fibrotest-Actitest. A mutation of TP53 at codon 249 (R249S), specific for exposure to aflatoxin, was detected in cell-free DNA extracted from plasma. Results: Overall, 276 subjects were HBsAg-positive (18.8%). Among 152 subjects tested for HBV load, 49 (32.2%) had over 104 copies/mL and 16 (10.5%) had levels below the limit of detection. The E genotype was found in 91.1% of carriers. Fibrotest scores ≥ F2 were observed in 52 subjects (35.4%). Actitest scores ≥ A2 were detected in 15 subjects (10.2%) and were correlated with Fibrotest scores (p = 0.0006). Among 105 subjects tested, 60% had detectable levels of R249S copies (>40 copies/mL plasma). Conclusion: Chronic HB carriage in adults in Bamako district is well over epidemic threshold. About 1/3 of carriers have moderate to severe liver fibrosis and 60% have detectable aflatoxin-related TP53 R249S mutation. These results support introduction of anti-HB therapies to reduce the progression towards severe liver disease.

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