Molecular biology of circulatory shock. Part II. Expression of four groups of hepatic genes is enhanced after resuscitation from cardiogenic shock

T. G. Buchman, D. E. Cabin, S. Vickers, C. S. Deutschman, E. Delgado, Marc S Sussman, G. B. Bulkley

Research output: Contribution to journalArticle

Abstract

Despite an initially successful resuscitation from circulatory shock, multiple organ failure (MOF) develops in some patients. The marked biochemical alterations associated with shock and MOF include clinically important changes in gene expression, such as altered rates of albumin and procoagulant synthesis. To characterize the MOF-associated changes at the cellular level, sequential liver biopsies were obtained from a swine model of cardiogenic shock associated with MOF. Preshock and postresuscitation biopsies were used not only to create a complementary DNA (cDNA) library but also to screen, to confirm, and, in nine out of 12 cases, to specifically identify genes whose expression is enhanced at least fivefold after resuscitation. The twelve genes thus characterized can be separated according to function into distinct groups, including the acute-phase genes and the heat-shock genes. Expression of acute-phase genes is liver specific and is essential for systemic homeostasis; heat-shock gene expression is generic to all cells and important for intracellular homeostasis.

Original languageEnglish (US)
Pages (from-to)559-566
Number of pages8
JournalSurgery
Volume108
Issue number3
StatePublished - 1990

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Cardiogenic Shock
Multiple Organ Failure
Resuscitation
Molecular Biology
Shock
Liver
Gene Expression
Genes
Homeostasis
Hot Temperature
Biopsy
Gene Library
Albumins
Swine
Complementary DNA

ASJC Scopus subject areas

  • Surgery

Cite this

Buchman, T. G., Cabin, D. E., Vickers, S., Deutschman, C. S., Delgado, E., Sussman, M. S., & Bulkley, G. B. (1990). Molecular biology of circulatory shock. Part II. Expression of four groups of hepatic genes is enhanced after resuscitation from cardiogenic shock. Surgery, 108(3), 559-566.

Molecular biology of circulatory shock. Part II. Expression of four groups of hepatic genes is enhanced after resuscitation from cardiogenic shock. / Buchman, T. G.; Cabin, D. E.; Vickers, S.; Deutschman, C. S.; Delgado, E.; Sussman, Marc S; Bulkley, G. B.

In: Surgery, Vol. 108, No. 3, 1990, p. 559-566.

Research output: Contribution to journalArticle

Buchman, TG, Cabin, DE, Vickers, S, Deutschman, CS, Delgado, E, Sussman, MS & Bulkley, GB 1990, 'Molecular biology of circulatory shock. Part II. Expression of four groups of hepatic genes is enhanced after resuscitation from cardiogenic shock', Surgery, vol. 108, no. 3, pp. 559-566.
Buchman, T. G. ; Cabin, D. E. ; Vickers, S. ; Deutschman, C. S. ; Delgado, E. ; Sussman, Marc S ; Bulkley, G. B. / Molecular biology of circulatory shock. Part II. Expression of four groups of hepatic genes is enhanced after resuscitation from cardiogenic shock. In: Surgery. 1990 ; Vol. 108, No. 3. pp. 559-566.
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