Molecular aspects in feedback regulation of gene expression by cholesterol in mammalian cells

Timothy F. Osborne, Vickie J. LaMorte

Research output: Contribution to journalArticle

Abstract

Intracellular cholesterol balance is maintained by a tight feedback mechanism that prevents the overaccumulation of cholesterol to cytotoxic levels. This is achieved through the coordinate regulation of genes of cholesterol uptake and biosynthesis by the sterol regulatory element binding proteins (SREBPs). The SREBPs are synthesized as membrane bound precursors that are released from their membrane tether when the cell needs new cholesterol. In the present article we present a model for how the cholesterol uptake pathway may be activated before the biosynthetic pathway to prevent wasting cellular energy and carbon on unneeded synthesis. Then we introduce a system for analyzing the differential localization and cellular trafficking of the different SREBP isoforms that can be performed over time in living cells.

Original languageEnglish (US)
Pages (from-to)42-48
Number of pages7
JournalMethods: A Companion to Methods in Enzymology
Volume16
Issue number1
DOIs
StatePublished - Sep 1998
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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