Molecular and epidemiological characterization of canine Pseudomonas otitis using a prospective case-control study design

Daniel O. Morris, Meghan Davis, Brian S. Palmeiro, Kathleen O'Shea, Shelley C. Rankin

Research output: Contribution to journalArticle

Abstract

Background: Pseudomonas aeruginosa is an opportunistic pathogen of the canine ear canal and occupies aquatic habitats in the environment. Nosocomial and zoonotic transmission of P. aeruginosa have been documented, including clonal outbreaks. Hypothesis/Objectives: The primary objective of this study was to assess various environmental exposures as potential risk factors for canine Pseudomonas otitis. It was hypothesized that isolates derived from infected ears would be clonal to isolates derived from household water sources and the mouths of human and animal companions of the study subjects. Animals: Seventy seven privately owned dogs with otitis were enrolled, along with their human and animal household companions, in a case-control design. Methods: Data on potential risk factors for Pseudomonas otitis were collected. Oral cavities of all study subjects, their human and animal companions, and household water sources were sampled. Pulsed field gel electrophoresis was used to estimate clonal relatedness of P. aeruginosa isolates. Results: In a multivariate model, visiting a dog park was associated with 77% increased odds of case status (P = 0.048). Strains clonal to the infection isolates were obtained from subjects' mouths (n = 18), companion pets' mouths (n = 5), pet owners' mouths (n = 2), water bowls (n = 7) and water taps (n = 2). Clonally related P. aeruginosa isolates were obtained from dogs that had no clear epidemiological link. Conclusions and clinical importance: Genetic homology between otic and environmental isolates is consistent with a waterborne source for some dogs, and cross-contamination with other human and animal members within some households.

Original languageEnglish (US)
JournalVeterinary Dermatology
DOIs
StateAccepted/In press - 2016

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Otitis
Pets
Pseudomonas
case-control studies
Mouth
Canidae
Case-Control Studies
experimental design
Pseudomonas aeruginosa
mouth
pets
dogs
Dogs
households
Water
ears
Ear
risk factors
Ear Canal
Pulsed Field Gel Electrophoresis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular and epidemiological characterization of canine Pseudomonas otitis using a prospective case-control study design. / Morris, Daniel O.; Davis, Meghan; Palmeiro, Brian S.; O'Shea, Kathleen; Rankin, Shelley C.

In: Veterinary Dermatology, 2016.

Research output: Contribution to journalArticle

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abstract = "Background: Pseudomonas aeruginosa is an opportunistic pathogen of the canine ear canal and occupies aquatic habitats in the environment. Nosocomial and zoonotic transmission of P. aeruginosa have been documented, including clonal outbreaks. Hypothesis/Objectives: The primary objective of this study was to assess various environmental exposures as potential risk factors for canine Pseudomonas otitis. It was hypothesized that isolates derived from infected ears would be clonal to isolates derived from household water sources and the mouths of human and animal companions of the study subjects. Animals: Seventy seven privately owned dogs with otitis were enrolled, along with their human and animal household companions, in a case-control design. Methods: Data on potential risk factors for Pseudomonas otitis were collected. Oral cavities of all study subjects, their human and animal companions, and household water sources were sampled. Pulsed field gel electrophoresis was used to estimate clonal relatedness of P. aeruginosa isolates. Results: In a multivariate model, visiting a dog park was associated with 77{\%} increased odds of case status (P = 0.048). Strains clonal to the infection isolates were obtained from subjects' mouths (n = 18), companion pets' mouths (n = 5), pet owners' mouths (n = 2), water bowls (n = 7) and water taps (n = 2). Clonally related P. aeruginosa isolates were obtained from dogs that had no clear epidemiological link. Conclusions and clinical importance: Genetic homology between otic and environmental isolates is consistent with a waterborne source for some dogs, and cross-contamination with other human and animal members within some households.",
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