Molecular analysis of sleep: wake cycles in Drosophila

A. Sehgal, W. Joiner, A. Crocker, K. Koh, S. Sathyanarayanan, Y. Fang, Mark Wu, J. A. Williams, X. Zheng

Research output: Contribution to journalArticle

Abstract

Sleep is controlled by two major regulatory systems: a circadian system that drives it with a 24-hour periodicity and a homeostatic system that ensures that adequate amounts of sleep are obtained. We are using the fruit fly Drosophila melanogaster to understand both types of regulation. With respect to circadian control, we have identified molecular mechanisms that are critical for the generation of a clock. Our recent efforts have focused on the analysis of posttranslational mechanisms, specifically the action of different phosphatases that control the phosphorylation and thereby the stability and/or nuclear localization of circadian clock proteins period (PER) and timeless (TIM). Resetting the clock in response to light is also mediated through posttranslational events that target TIM for degradation by the proteasome pathway; a recently identified ubiquitin ligase, jet lag (JET), is required for this response. Our understanding of the homeostatic control of sleep is in its early stages. We have found that mushroom bodies, which are a site of synaptic plasticity in the fly brain, are important for the regulation of sleep. In addition, through analysis of genes expressed under different behavioral states, we have identified some that are up-regulated during sleep deprivation. Thus, the Drosophila model allows the use of cellular and molecular approaches that should ultimately lead to a better understanding of sleep biology.

Original languageEnglish (US)
Pages (from-to)557-564
Number of pages8
JournalCold Spring Harbor Symposia on Quantitative Biology
Volume72
DOIs
StatePublished - 2007
Externally publishedYes

Fingerprint

sleep
Drosophila
Sleep
Diptera
Period Circadian Proteins
Clocks
Mushroom Bodies
mushroom bodies
Neuronal Plasticity
Sleep Deprivation
proteasome endopeptidase complex
Periodicity
Proteasome Endopeptidase Complex
Ligases
ubiquitin
fruit flies
Ubiquitin
Phosphorylation
Drosophila melanogaster
ligases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Biochemistry
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sehgal, A., Joiner, W., Crocker, A., Koh, K., Sathyanarayanan, S., Fang, Y., ... Zheng, X. (2007). Molecular analysis of sleep: wake cycles in Drosophila. Cold Spring Harbor Symposia on Quantitative Biology, 72, 557-564. https://doi.org/10.1101/sqb.2007.72.018

Molecular analysis of sleep : wake cycles in Drosophila. / Sehgal, A.; Joiner, W.; Crocker, A.; Koh, K.; Sathyanarayanan, S.; Fang, Y.; Wu, Mark; Williams, J. A.; Zheng, X.

In: Cold Spring Harbor Symposia on Quantitative Biology, Vol. 72, 2007, p. 557-564.

Research output: Contribution to journalArticle

Sehgal, A, Joiner, W, Crocker, A, Koh, K, Sathyanarayanan, S, Fang, Y, Wu, M, Williams, JA & Zheng, X 2007, 'Molecular analysis of sleep: wake cycles in Drosophila', Cold Spring Harbor Symposia on Quantitative Biology, vol. 72, pp. 557-564. https://doi.org/10.1101/sqb.2007.72.018
Sehgal, A. ; Joiner, W. ; Crocker, A. ; Koh, K. ; Sathyanarayanan, S. ; Fang, Y. ; Wu, Mark ; Williams, J. A. ; Zheng, X. / Molecular analysis of sleep : wake cycles in Drosophila. In: Cold Spring Harbor Symposia on Quantitative Biology. 2007 ; Vol. 72. pp. 557-564.
@article{44eab06e45814e9abbdd6790b1005649,
title = "Molecular analysis of sleep: wake cycles in Drosophila",
abstract = "Sleep is controlled by two major regulatory systems: a circadian system that drives it with a 24-hour periodicity and a homeostatic system that ensures that adequate amounts of sleep are obtained. We are using the fruit fly Drosophila melanogaster to understand both types of regulation. With respect to circadian control, we have identified molecular mechanisms that are critical for the generation of a clock. Our recent efforts have focused on the analysis of posttranslational mechanisms, specifically the action of different phosphatases that control the phosphorylation and thereby the stability and/or nuclear localization of circadian clock proteins period (PER) and timeless (TIM). Resetting the clock in response to light is also mediated through posttranslational events that target TIM for degradation by the proteasome pathway; a recently identified ubiquitin ligase, jet lag (JET), is required for this response. Our understanding of the homeostatic control of sleep is in its early stages. We have found that mushroom bodies, which are a site of synaptic plasticity in the fly brain, are important for the regulation of sleep. In addition, through analysis of genes expressed under different behavioral states, we have identified some that are up-regulated during sleep deprivation. Thus, the Drosophila model allows the use of cellular and molecular approaches that should ultimately lead to a better understanding of sleep biology.",
author = "A. Sehgal and W. Joiner and A. Crocker and K. Koh and S. Sathyanarayanan and Y. Fang and Mark Wu and Williams, {J. A.} and X. Zheng",
year = "2007",
doi = "10.1101/sqb.2007.72.018",
language = "English (US)",
volume = "72",
pages = "557--564",
journal = "Cold Spring Harbor Symposia on Quantitative Biology",
issn = "0091-7451",
publisher = "Cold Spring Harbor Laboratory Press",

}

TY - JOUR

T1 - Molecular analysis of sleep

T2 - wake cycles in Drosophila

AU - Sehgal, A.

AU - Joiner, W.

AU - Crocker, A.

AU - Koh, K.

AU - Sathyanarayanan, S.

AU - Fang, Y.

AU - Wu, Mark

AU - Williams, J. A.

AU - Zheng, X.

PY - 2007

Y1 - 2007

N2 - Sleep is controlled by two major regulatory systems: a circadian system that drives it with a 24-hour periodicity and a homeostatic system that ensures that adequate amounts of sleep are obtained. We are using the fruit fly Drosophila melanogaster to understand both types of regulation. With respect to circadian control, we have identified molecular mechanisms that are critical for the generation of a clock. Our recent efforts have focused on the analysis of posttranslational mechanisms, specifically the action of different phosphatases that control the phosphorylation and thereby the stability and/or nuclear localization of circadian clock proteins period (PER) and timeless (TIM). Resetting the clock in response to light is also mediated through posttranslational events that target TIM for degradation by the proteasome pathway; a recently identified ubiquitin ligase, jet lag (JET), is required for this response. Our understanding of the homeostatic control of sleep is in its early stages. We have found that mushroom bodies, which are a site of synaptic plasticity in the fly brain, are important for the regulation of sleep. In addition, through analysis of genes expressed under different behavioral states, we have identified some that are up-regulated during sleep deprivation. Thus, the Drosophila model allows the use of cellular and molecular approaches that should ultimately lead to a better understanding of sleep biology.

AB - Sleep is controlled by two major regulatory systems: a circadian system that drives it with a 24-hour periodicity and a homeostatic system that ensures that adequate amounts of sleep are obtained. We are using the fruit fly Drosophila melanogaster to understand both types of regulation. With respect to circadian control, we have identified molecular mechanisms that are critical for the generation of a clock. Our recent efforts have focused on the analysis of posttranslational mechanisms, specifically the action of different phosphatases that control the phosphorylation and thereby the stability and/or nuclear localization of circadian clock proteins period (PER) and timeless (TIM). Resetting the clock in response to light is also mediated through posttranslational events that target TIM for degradation by the proteasome pathway; a recently identified ubiquitin ligase, jet lag (JET), is required for this response. Our understanding of the homeostatic control of sleep is in its early stages. We have found that mushroom bodies, which are a site of synaptic plasticity in the fly brain, are important for the regulation of sleep. In addition, through analysis of genes expressed under different behavioral states, we have identified some that are up-regulated during sleep deprivation. Thus, the Drosophila model allows the use of cellular and molecular approaches that should ultimately lead to a better understanding of sleep biology.

UR - http://www.scopus.com/inward/record.url?scp=48249094942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48249094942&partnerID=8YFLogxK

U2 - 10.1101/sqb.2007.72.018

DO - 10.1101/sqb.2007.72.018

M3 - Article

C2 - 18419315

AN - SCOPUS:48249094942

VL - 72

SP - 557

EP - 564

JO - Cold Spring Harbor Symposia on Quantitative Biology

JF - Cold Spring Harbor Symposia on Quantitative Biology

SN - 0091-7451

ER -