Molecular analysis of ovarian mucinous carcinoma reveals different cell of origins

Yihong Wang, Lauren Ende Shwartz, Derek Anderson, Ming-Tseh Lin, Lisa Haley, Ren chin Wu, Russell S Vang, Ie Ming Shih, Robert J Kurman

Research output: Contribution to journalArticle

Abstract

It is believed that a subset of primary ovarian mucinous tumors is derived from mature teratomas [1-5]. To confirm this, we performed microsatellite genotyping using a variety of short tandem repeat makers and analyzed allelotypes of 8 mucinous tumors (4 mucinous carcinomas, 3 atypical proliferative mucinous tumors and 1 mucinous cystadenoma) associated with a teratoma to determine whether they were clonally related. 7 of the 8 mucinous tumors showed complete or a high degree of homozygosity. Among the 6 pairs of tumors with teratoma tissue available for comparison, 5 of 6 showed a high or complete degree of allelotypes matching, which differed from the somatic allelotypes of the normal control tissue. A discrepancy was detected between carcinoma and teratoma in one pair at several loci, with different X-chromosome inactivation patterns revealed by the HUMARA clonality assay. We also investigated the allelotypes of 16 ovarian mucinous carcinomas without a teratoma in young patients (range 13-30) and in 6 older patients (range 31-67) using the same method. None of these tumors showed pure homozygosity. The number of homozygous loci in this cohort was significantly lower than that in the first. Our results suggest first, that most mucinous tumors associated with a teratoma are derived from the teratoma but occasionally they could be collision tumors and second that the majority of pure mucinous tumors in young women in whom a teratoma is not present are not derived from a teratoma.

Original languageEnglish (US)
Pages (from-to)22949-22958
Number of pages10
JournalOncotarget
Volume6
Issue number26
StatePublished - 2015

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Mucinous Adenocarcinoma
Teratoma
Neoplasms
Microsatellite Repeats
Mucinous Cystadenoma
X Chromosome Inactivation
Carcinoma

Keywords

  • HUMARA assay
  • Microsatellite genotyping
  • Mucinous carcinoma
  • Ovarian
  • Teratoma

ASJC Scopus subject areas

  • Oncology

Cite this

Wang, Y., Shwartz, L. E., Anderson, D., Lin, M-T., Haley, L., Wu, R. C., ... Kurman, R. J. (2015). Molecular analysis of ovarian mucinous carcinoma reveals different cell of origins. Oncotarget, 6(26), 22949-22958.

Molecular analysis of ovarian mucinous carcinoma reveals different cell of origins. / Wang, Yihong; Shwartz, Lauren Ende; Anderson, Derek; Lin, Ming-Tseh; Haley, Lisa; Wu, Ren chin; Vang, Russell S; Shih, Ie Ming; Kurman, Robert J.

In: Oncotarget, Vol. 6, No. 26, 2015, p. 22949-22958.

Research output: Contribution to journalArticle

Wang, Y, Shwartz, LE, Anderson, D, Lin, M-T, Haley, L, Wu, RC, Vang, RS, Shih, IM & Kurman, RJ 2015, 'Molecular analysis of ovarian mucinous carcinoma reveals different cell of origins', Oncotarget, vol. 6, no. 26, pp. 22949-22958.
Wang Y, Shwartz LE, Anderson D, Lin M-T, Haley L, Wu RC et al. Molecular analysis of ovarian mucinous carcinoma reveals different cell of origins. Oncotarget. 2015;6(26):22949-22958.
Wang, Yihong ; Shwartz, Lauren Ende ; Anderson, Derek ; Lin, Ming-Tseh ; Haley, Lisa ; Wu, Ren chin ; Vang, Russell S ; Shih, Ie Ming ; Kurman, Robert J. / Molecular analysis of ovarian mucinous carcinoma reveals different cell of origins. In: Oncotarget. 2015 ; Vol. 6, No. 26. pp. 22949-22958.
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