Molecular analysis of erection regulatory factors in sickle cell disease associated priapism in the human penis

Gwen Lagoda, Sena F. Sezen, Marcelo R. Cabrini, Biljana Musicki, Arthur Burnett

Research output: Contribution to journalArticle

Abstract

Purpose: Priapism is a vasculopathy that occurs in approximately 40% of patients with sickle cell disease. Mouse models suggest that dysregulated nitric oxide synthase and RhoA/ROCK signaling as well as increased oxidative stress may contribute to the mechanisms of sickle cell disease associated priapism. We examined changes in the protein expression of nitric oxide synthase and ROCK signaling pathways, and a source of oxidative stress, NADPH oxidase, in penile erectile tissue from patients with a priapism history etiologically related and unrelated to sickle cell disease. Materials and Methods: Human penile erectile tissue was obtained from 5 patients with sickle cell disease associated priapism and from 6 with priapism of other etiologies during nonemergent penile prosthesis surgery for erectile dysfunction or priapism management and urethroplasty. Tissue was also obtained from 5 control patients without a priapism history during penectomy for penile cancer. Samples were collected, immediately placed in cold buffer and then frozen in liquid nitrogen. The expression of phosphodiesterase 5, endothelial nitric oxide synthase, neuronal nitric oxide synthase, inducible nitric oxide synthase, RhoA, ROCK1, ROCK2, p47phox, p67phox, gp91phox and β-actin were determined by Western blot analysis. Nitric oxide was measured using the Griess reaction. Results: In the sickle cell disease group phosphodiesterase 5 (p phox expression (p phox expression (each p

Original languageEnglish (US)
Pages (from-to)762-768
Number of pages7
JournalJournal of Urology
Volume189
Issue number2
DOIs
StatePublished - Feb 2013

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Priapism
Penis
Sickle Cell Anemia
Type 5 Cyclic Nucleotide Phosphodiesterases
Nitric Oxide Synthase
Oxidative Stress
Penile Neoplasms
Penile Prosthesis
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type III
NADPH Oxidase
Nitric Oxide Synthase Type II
Erectile Dysfunction
Actins
Buffers
Nitric Oxide
Nitrogen
Western Blotting

Keywords

  • anemia
  • nitric oxide
  • oxidative stress
  • penis
  • priapism
  • sickle cell

ASJC Scopus subject areas

  • Urology

Cite this

Molecular analysis of erection regulatory factors in sickle cell disease associated priapism in the human penis. / Lagoda, Gwen; Sezen, Sena F.; Cabrini, Marcelo R.; Musicki, Biljana; Burnett, Arthur.

In: Journal of Urology, Vol. 189, No. 2, 02.2013, p. 762-768.

Research output: Contribution to journalArticle

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