Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin

Chontida Tangsongcharoen, Natapong Jupatanakul, Boonhiang Promdonkoy, George Dimopoulos, Panadda Boonserm

Research output: Contribution to journalArticle

Abstract

Lysinibacillus sphaericus produces the mosquito larvicidal binary toxin consisting of BinA and BinB, which are both required for toxicity against Culex and Anopheles larvae. The molecular mechanisms behind Bin toxin-induced damage remain unexplored. We used whole-genome microarray-based transcriptome analysis to better understand how Culex larvae respond to Bin toxin treatment at the molecular level. Our analyses of Culex quinquefasciatus larvae transcriptome changes at 6, 12, and 18 h after Bin toxin treatment revealed a wide range of transcript signatures, including genes linked to the cytoskeleton, metabolism, immunity, and cellular stress, with a greater number of down-regulated genes than up-regulated genes. Bin toxin appears to mainly repress the expression of genes involved in metabolism, the mitochondrial electron transport chain, and the protein transporter of the outer/inner mitochondrial membrane. The induced genes encode proteins linked to mitochondrial-mediated apoptosis and cellular detoxification including autophagic processes and lysosomal compartments. This study is, to our knowledge, the first microarray analysis of Bin toxin-induced transcriptional responses in Culex larvae, providing a basis for an in-depth understanding of the molecular nature of Bin toxin-induced damage.

Original languageEnglish (US)
Article numbere0175473
JournalPLoS ONE
Volume12
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Culex
Larva
toxins
Bins
Cyclic AMP Receptor Protein
Genes
larvae
Mitochondrial Membranes
genes
Metabolism
Canidae
Anopheles
Gene Expression Profiling
Microarray Analysis
Electron Transport
Culicidae
Cytoskeleton
Transcriptome
Cellular Immunity
Carrier Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tangsongcharoen, C., Jupatanakul, N., Promdonkoy, B., Dimopoulos, G., & Boonserm, P. (2017). Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin. PLoS ONE, 12(4), [e0175473]. DOI: 10.1371/journal.pone.0175473

Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin. / Tangsongcharoen, Chontida; Jupatanakul, Natapong; Promdonkoy, Boonhiang; Dimopoulos, George; Boonserm, Panadda.

In: PLoS ONE, Vol. 12, No. 4, e0175473, 01.04.2017.

Research output: Contribution to journalArticle

Tangsongcharoen, C, Jupatanakul, N, Promdonkoy, B, Dimopoulos, G & Boonserm, P 2017, 'Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin' PLoS ONE, vol 12, no. 4, e0175473. DOI: 10.1371/journal.pone.0175473
Tangsongcharoen C, Jupatanakul N, Promdonkoy B, Dimopoulos G, Boonserm P. Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin. PLoS ONE. 2017 Apr 1;12(4). e0175473. Available from, DOI: 10.1371/journal.pone.0175473

Tangsongcharoen, Chontida; Jupatanakul, Natapong; Promdonkoy, Boonhiang; Dimopoulos, George; Boonserm, Panadda / Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin.

In: PLoS ONE, Vol. 12, No. 4, e0175473, 01.04.2017.

Research output: Contribution to journalArticle

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