Molecular analysis of a pro‐T cell clone transformed by Abelson‐murine leukemia virus, displaying progressive γδ T cell receptor gene rearrangement and surface expression

Françoise Heuze, Drew Pardoll, Elisabeth Diez, Sophie Ezine, Francine Jotereau

Research output: Contribution to journalArticlepeer-review

Abstract

We present a molecular analysis of T cell differentiation in a set of clones derived from in vitro Abelson murine leukemia virus (A‐MuLV) infection of fetal liver cells. The parental clone had partial rearrangement of the β and γ loci and spontaneously displayed progressive rearrangement of Vγ genes during in vitro culture. Further differentiation of these clones leading to δ gene rearrangement and CD4 expression, then CD8, CD3 and T cell receptor γδ chain surface expression was obtained after intrathymic transfer followed by in vitro co‐culture with thymic tissue. These A‐MuLV clones, therefore, appear to represent a powerful model sytem for studying the early molecular events of T cell development at the clonal level.

Original languageEnglish (US)
Pages (from-to)2077-2084
Number of pages8
JournalEuropean Journal of Immunology
Volume22
Issue number8
DOIs
StatePublished - Aug 1992

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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