Molecular analysis of a pro-T cell clone transformed by Abelson-murine leukemia virus, displaying progressive γδ T cell receptor gene rearrangement and surface expression

Françoise Heuze, Andrew Mark Pardoll, Elisabeth Diez, Sophie Ezine, Francine Jotereau

Research output: Contribution to journalArticle

Abstract

We present a molecular analysis of T cell differentiation in a set of clones derived from in vitro Abelson murine leukemia virus (A-MuLV) infection of fetal liver cells. The parental clone had partial rearrangement of the β and γ loci and spontaneously displayed progressive rearrangement of Vγ genes during in vitro culture. Further differentiation of these clones leading to δ gene rearrangement and CD4 expression, then CD8, CD3 and T cell receptor γδ chain surface expression was obtained after intrathymic transfer followed by in vitro co-culture with thymic tissue. These A-MuLV clones, therefore, appear to represent a powerful model sytem for studying the early molecular events of T cell development at the clonal level.

Original languageEnglish (US)
Pages (from-to)2077-2084
Number of pages8
JournalEuropean Journal of Immunology
Volume22
Issue number8
StatePublished - Aug 1992

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Abelson murine leukemia virus
T-Lymphocyte Gene Rearrangement
T-Cell Receptor Genes
Clone Cells
T-Lymphocytes
Gene Rearrangement
Virus Diseases
Coculture Techniques
T-Cell Antigen Receptor
Cell Differentiation
Liver
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular analysis of a pro-T cell clone transformed by Abelson-murine leukemia virus, displaying progressive γδ T cell receptor gene rearrangement and surface expression. / Heuze, Françoise; Pardoll, Andrew Mark; Diez, Elisabeth; Ezine, Sophie; Jotereau, Francine.

In: European Journal of Immunology, Vol. 22, No. 8, 08.1992, p. 2077-2084.

Research output: Contribution to journalArticle

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