TY - JOUR
T1 - Molecular amplification assays to detect chlamydial infections in urine specimens from high school female students and to monitor the persistence of chlamydial DNA after therapy
AU - Gaydos, Charlotte A.
AU - Crotchfelt, Kimberly A.
AU - Howell, M. Rene
AU - Kralian, Susan
AU - Hauptman, Patricia
AU - Quinn, Thomas C.
N1 - Funding Information:
Infectious Disease Division, Johns Hopkins University, and Baltimore City Health Department, Baltimore, and National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland
Funding Information:
Financial support: Region III Chlamydia Program (CDC); Roche Molecular Systems (Branchburg, NJ) and Abbott Laboratories (Abbott Park, IL) donated test kits.
PY - 1998
Y1 - 1998
N2 - Polymerase chain reaction (PCR) and ligase chain reaction (LCR) were compared for the diagnosis of Chlamydia trachomatis infections by testing urine specimens from 408 high school female students. After therapy, sequential urine specimens were tested to determine persistence of chlamydial DNA in urine. Baseline PCR of cervical specimens was positive in 53 (13.0%) students, and PCR and LCR of urine specimens were positive in 63 (15.4%) and 60 (14.7%), respectively. After discrepant analysis, 64 (15.7%) patients could be confirmed as truly infected. Follow-up urine specimens from 33 infected patients demonstrated that at 1-3 days after therapy, PCR and LCR were positive for 40% and 73.3%, respectively. Only at 15 days after therapy did all specimens test negative. Urine tests for Chlamydia organisms should not be used as a test of cure within 3 weeks after treatment. Use of urine assays for screening sexually active adolescents has the potential to significantly improve control of chlamydial infections.
AB - Polymerase chain reaction (PCR) and ligase chain reaction (LCR) were compared for the diagnosis of Chlamydia trachomatis infections by testing urine specimens from 408 high school female students. After therapy, sequential urine specimens were tested to determine persistence of chlamydial DNA in urine. Baseline PCR of cervical specimens was positive in 53 (13.0%) students, and PCR and LCR of urine specimens were positive in 63 (15.4%) and 60 (14.7%), respectively. After discrepant analysis, 64 (15.7%) patients could be confirmed as truly infected. Follow-up urine specimens from 33 infected patients demonstrated that at 1-3 days after therapy, PCR and LCR were positive for 40% and 73.3%, respectively. Only at 15 days after therapy did all specimens test negative. Urine tests for Chlamydia organisms should not be used as a test of cure within 3 weeks after treatment. Use of urine assays for screening sexually active adolescents has the potential to significantly improve control of chlamydial infections.
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U2 - 10.1086/514207
DO - 10.1086/514207
M3 - Article
C2 - 9466530
AN - SCOPUS:0031883485
SN - 0022-1899
VL - 177
SP - 417
EP - 424
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -