Modulation of wound healing and scar formation by MG53 protein-mediated cell membrane repair

Haichang Li, Pu Duann, Pei Hui Lin, Li Zhao, Zhaobo Fan, Tao Tan, Xinyu Zhou, Mingzhai Sun, Minghuan Fu, Matthew Orange, Matthew Sermersheim, Hanley Ma, Duofen He, Steven M. Steinberg, Robert Higgins, Hua Zhu, Elizabeth John, Chunyu Zeng, Jianjun Guan, Jianjie Ma

Research output: Contribution to journalArticle

Abstract

Cell membrane repair is an important aspect of physiology, and disruption of this process can result in pathophysiology in a number of different tissues, including wound healing, chronic ulcer and scarring. We have previously identified a novel tripartite motif family protein, MG53, as an essential component of the cell membrane repair machinery. Here we report the functional role of MG53 in the modulation of wound healing and scarring. Although MG53 is absent from keratinocytes and fibroblasts, remarkable defects in skin architecture and collagen overproduction are observed in mg53-/- mice, and these animals display delayed wound healing and abnormal scarring. Recombinant human MG53 (rhMG53) protein, encapsulated in a hydrogel formulation, facilitates wound healing and prevents scarring in rodent models of dermal injuries. An in vitro study shows that rhMG53 protects against acute injury to keratinocytes and facilitates the migration of fibroblasts in response to scratch wounding. During fibrotic remodeling, rhMG53 interferes with TGF-β-dependent activation of myofibroblast differentiation. The resulting down-regulation of α smooth muscle actin and extracellular matrix proteins contributes to reduced scarring. Overall, these studies establish a trifunctional role for MG53 as a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing. Targeting the functional interaction between MG53 and TGF-β signaling may present a potentially effective means for promoting scarless wound healing.

Original languageEnglish (US)
Pages (from-to)24592-24603
Number of pages12
JournalJournal of Biological Chemistry
Volume290
Issue number40
DOIs
StatePublished - Oct 2 2015
Externally publishedYes

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Cell membranes
Wound Healing
Cicatrix
Membrane Proteins
Repair
Modulation
Fibroblasts
Proteins
Myofibroblasts
Extracellular Matrix Proteins
Hydrogel
Physiology
Keratinocytes
Modulators
Machinery
Muscle
Actins
Skin
Wounds and Injuries
Animals

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Modulation of wound healing and scar formation by MG53 protein-mediated cell membrane repair. / Li, Haichang; Duann, Pu; Lin, Pei Hui; Zhao, Li; Fan, Zhaobo; Tan, Tao; Zhou, Xinyu; Sun, Mingzhai; Fu, Minghuan; Orange, Matthew; Sermersheim, Matthew; Ma, Hanley; He, Duofen; Steinberg, Steven M.; Higgins, Robert; Zhu, Hua; John, Elizabeth; Zeng, Chunyu; Guan, Jianjun; Ma, Jianjie.

In: Journal of Biological Chemistry, Vol. 290, No. 40, 02.10.2015, p. 24592-24603.

Research output: Contribution to journalArticle

Li, H, Duann, P, Lin, PH, Zhao, L, Fan, Z, Tan, T, Zhou, X, Sun, M, Fu, M, Orange, M, Sermersheim, M, Ma, H, He, D, Steinberg, SM, Higgins, R, Zhu, H, John, E, Zeng, C, Guan, J & Ma, J 2015, 'Modulation of wound healing and scar formation by MG53 protein-mediated cell membrane repair', Journal of Biological Chemistry, vol. 290, no. 40, pp. 24592-24603. https://doi.org/10.1074/jbc.M115.680074
Li, Haichang ; Duann, Pu ; Lin, Pei Hui ; Zhao, Li ; Fan, Zhaobo ; Tan, Tao ; Zhou, Xinyu ; Sun, Mingzhai ; Fu, Minghuan ; Orange, Matthew ; Sermersheim, Matthew ; Ma, Hanley ; He, Duofen ; Steinberg, Steven M. ; Higgins, Robert ; Zhu, Hua ; John, Elizabeth ; Zeng, Chunyu ; Guan, Jianjun ; Ma, Jianjie. / Modulation of wound healing and scar formation by MG53 protein-mediated cell membrane repair. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 40. pp. 24592-24603.
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AU - Zhao, Li

AU - Fan, Zhaobo

AU - Tan, Tao

AU - Zhou, Xinyu

AU - Sun, Mingzhai

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AU - Orange, Matthew

AU - Sermersheim, Matthew

AU - Ma, Hanley

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AU - Steinberg, Steven M.

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