Modulation of ultraviolet-induced hyperalgesia and cytokine upregulation by interleukins 10 and 13

Nayef E. Saadé, Isam Nasr, Cynthia A. Massaad, Bared Safieh-Garabedian, Suhayl J. Jabbur, Salim A. Kanaan

Research output: Contribution to journalArticle

Abstract

1. Exposure to midrange ultraviolet radiation (UVB) is known to produce skin inflammation similar to sunburn. The aim of this study was to characterize the hyperalgesia and cytokine upregulation induced by UVB and their modulation by antiinflammatory cytokines. 2. Acute exposure of the dorsal skin of mice to UVB (200, 250 and 300 mJ cm 2) resulted in a dose-dependent decrease in the latencies of the hot plate and tail flick tests, without evident signs of skin lesions. 3. The observed hyperalgesia displayed a biphasic temporal evolution with an acute phase (3-6 h) and a late (48-96 h) phase. 4. Exposure to UVB (300 mJ cm 2) elicited significant upregulation of interleukin (IL)-1β, tumour necrosis factor (TNF)-α and nerve growth factor (NGF), determined by ELISA in the exposed skin. This upregulation was more important during the acute phase of hyperalgesia. 5. Daily treatment of mice, with i.p. injections of either IL-10 or IL-13 (1.5, 7.5 and 15 ng in 100 μl saline) produced a dose-dependent attenuation of the UVB-induced hyperalgesia. 6. Treatment with the highest doses of either IL-10 or IL-13, produced significant attenuation of the levels of the cytokines and NGF by UVB, with relatively more pronounced effects by IL-13. 7. Acute exposure to moderate amounts of UVB results in a systemic hyperalgesia related to the upregulation of cytokine and NGF levels, since both were prevented by treatment with antiinflammatory cytokines.

Original languageEnglish (US)
Pages (from-to)1317-1324
Number of pages8
JournalBritish Journal of Pharmacology
Volume131
Issue number7
StatePublished - 2000
Externally publishedYes

Fingerprint

Interleukin-13
Hyperalgesia
Interleukin-10
Up-Regulation
Cytokines
Nerve Growth Factor
Skin
Anti-Inflammatory Agents
Sunburn
Interleukin-15
Interleukin-1
Tail
Therapeutics
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Radiation
Inflammation
Injections

Keywords

  • Inflammatory hyperalgesia
  • Interleukins
  • NGF
  • Pain-tests
  • TNF-α
  • Ultraviolet radiations

ASJC Scopus subject areas

  • Pharmacology

Cite this

Saadé, N. E., Nasr, I., Massaad, C. A., Safieh-Garabedian, B., Jabbur, S. J., & Kanaan, S. A. (2000). Modulation of ultraviolet-induced hyperalgesia and cytokine upregulation by interleukins 10 and 13. British Journal of Pharmacology, 131(7), 1317-1324.

Modulation of ultraviolet-induced hyperalgesia and cytokine upregulation by interleukins 10 and 13. / Saadé, Nayef E.; Nasr, Isam; Massaad, Cynthia A.; Safieh-Garabedian, Bared; Jabbur, Suhayl J.; Kanaan, Salim A.

In: British Journal of Pharmacology, Vol. 131, No. 7, 2000, p. 1317-1324.

Research output: Contribution to journalArticle

Saadé, NE, Nasr, I, Massaad, CA, Safieh-Garabedian, B, Jabbur, SJ & Kanaan, SA 2000, 'Modulation of ultraviolet-induced hyperalgesia and cytokine upregulation by interleukins 10 and 13', British Journal of Pharmacology, vol. 131, no. 7, pp. 1317-1324.
Saadé NE, Nasr I, Massaad CA, Safieh-Garabedian B, Jabbur SJ, Kanaan SA. Modulation of ultraviolet-induced hyperalgesia and cytokine upregulation by interleukins 10 and 13. British Journal of Pharmacology. 2000;131(7):1317-1324.
Saadé, Nayef E. ; Nasr, Isam ; Massaad, Cynthia A. ; Safieh-Garabedian, Bared ; Jabbur, Suhayl J. ; Kanaan, Salim A. / Modulation of ultraviolet-induced hyperalgesia and cytokine upregulation by interleukins 10 and 13. In: British Journal of Pharmacology. 2000 ; Vol. 131, No. 7. pp. 1317-1324.
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