Modulation of the transient outward current in adult rat ventricular myocytes by polyunsaturated fatty acids

K. Y. Bogdanov, H. A. Spurgeon, T. M. Vinogradova, E. G. Lakatta

Research output: Contribution to journalArticlepeer-review

Abstract

With the whole cell patch-clamp technique, we studied the effects of the n-3 and n-6 polyunsaturated fatty acids (PUFAs), linoleic (C18:2n-6), eicosapentaenoic (C20:4n-3), docosahexaenoic (C22:5n-3), and arachidonic (AA; C20:4n-6) acids, on K+ currents in rat ventricular myocytes. At low concentrations (5-10 μM) all PUFAs except AA inhibited, by ∼40%, the transient outward current (Ito) without affecting other K+ currents and markedly prolonged the action potential (AP). AA inhibited Ito but also augmented a sustained depolarization-induced outward K+ current (Isus); the latter effect did not occur in the presence of 4-aminopyridine or with eicosatetraynoic acid, a nonmetabolizable analog of AA. Higher concentrations of PUFAs (20-50 μM) further inhibited Ito and also inhibited Isus. Thus, at high concentrations, PUFAs have a nonspecific effect on several K+ channels; at low concentrations, PUFAs preferentially inhibit Ito and prolong the AP.

Original languageEnglish (US)
Pages (from-to)H571-H579
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume274
Issue number2 43-2
DOIs
StatePublished - Feb 1998
Externally publishedYes

Keywords

  • Arachidonic acid
  • Eicosapentaenoic acid
  • Fish oil
  • Linoleic acid
  • Potassium currents

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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