Abstract
Anti-ras oligodeoxyribonucleoside methylphosphonates (ONMPs) complementary to the initiation codon region have been synthesized to explore their efficacy and specificity on ras-p21 translation. ONMP (IC-0) precisely complementing the first initial 11 nucleotides of the Balb-ras initiation codon region acts in a dose-dependent manner to inhibit p21 translation by a rabbit reticulocyte lysate. At 100 μM, IC-0 inhibits the cell-free translation of p21 close to completion. The two control oligomers containing one or two nucleotide mismatches were significantly less effective than IC-0 at the equivalent concentration. In living cells, a perfectly matched ONMP directed against the initiation codon region inhibited Ha-ras p21 expression by 90% at a concentration of 50 μM.
Original language | English (US) |
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Pages (from-to) | 243-252 |
Number of pages | 10 |
Journal | Oncogene Research |
Volume | 4 |
Issue number | 4 |
State | Published - 1989 |
Externally published | Yes |
ASJC Scopus subject areas
- Cancer Research