Modulation of ras expression by anti-sense, nonionic deoxyoligonucleotide analogs

D. Brown, Z. Yu, P. Miller, K. Blake, C. Wei, H. F. Kung, R. J. Black, P. O.P. Ts'O, E. H. Chang

Research output: Contribution to journalArticlepeer-review

Abstract

Anti-ras oligodeoxyribonucleoside methylphosphonates (ONMPs) complementary to the initiation codon region have been synthesized to explore their efficacy and specificity on ras-p21 translation. ONMP (IC-0) precisely complementing the first initial 11 nucleotides of the Balb-ras initiation codon region acts in a dose-dependent manner to inhibit p21 translation by a rabbit reticulocyte lysate. At 100 μM, IC-0 inhibits the cell-free translation of p21 close to completion. The two control oligomers containing one or two nucleotide mismatches were significantly less effective than IC-0 at the equivalent concentration. In living cells, a perfectly matched ONMP directed against the initiation codon region inhibited Ha-ras p21 expression by 90% at a concentration of 50 μM.

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalOncogene Research
Volume4
Issue number4
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Cancer Research

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