Modulation of keratocyte phenotype by collagen fibril nanoarchitecture in membranes for corneal repair

Qiongyu Guo, Jude M. Phillip, Shoumyo Majumdar, Pei Hsun Wu, Jiansu Chen, Xiomara Calderón-Colón, Oliver D Schein, Barbara J. Smith, Morgana M. Trexler, Denis Wirtz, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Type I collagen membranes with tailored fibril nanoarchitectures were fabricated through a vitrification processing, which mimicked, to a degree, the collagen maturation process of corneal stromal extracellular matrix invivo. Vitrification was performed at a controlled temperature of either 5°C or 39°C at a constant relative humidity of 40% for various time periods from 0.5wk up to 8wk. During vitrification, the vitrified collagen membranes (collagen vitrigels, CVs) exhibited a rapid growth in fibrillar density through the evaporation of water and an increase in fibrillar stiffness due to the formation of new and/or more-stable interactions. On the other hand, the collagen fibrils in CVs maintained their D-periodicity and showed no significant difference in fibrillar diameter, indicating preservation of the native states of the collagen fibrils during vitrification. Keratocyte phenotype was maintained on CVs to varying degrees that were strongly influenced by the collagen fibril nanoarchitectures. Specifically, the vitrification time of CVs mainly governed the keratocyte morphology, showing significant increases in the cell protrusion number, protrusion length, and cell size along with CV vitrification time. The CV vitrification temperature affected the regulation of keratocyte fibroblasts' gene expressions, including keratocan and aldehyde dehydrogenase (ALDH), demonstrating a unique way to control the expression of specific genes invitro.

Original languageEnglish (US)
Pages (from-to)9365-9372
Number of pages8
JournalBiomaterials
Volume34
Issue number37
DOIs
StatePublished - Dec 2013

Fingerprint

Collagen
Vitrification
Repair
Modulation
Membranes
Phenotype
Gene Expression
Aldehyde Dehydrogenase
Temperature
Periodicity
Fibroblasts
Collagen Type I
Humidity
Cell Size
Gene expression
Aldehydes
Extracellular Matrix
Atmospheric humidity
Evaporation
Cell Count

Keywords

  • Collagen maturation
  • Corneal repair
  • Fibril nanoarchitecture
  • Keratocyte phenotype

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Modulation of keratocyte phenotype by collagen fibril nanoarchitecture in membranes for corneal repair. / Guo, Qiongyu; Phillip, Jude M.; Majumdar, Shoumyo; Wu, Pei Hsun; Chen, Jiansu; Calderón-Colón, Xiomara; Schein, Oliver D; Smith, Barbara J.; Trexler, Morgana M.; Wirtz, Denis; Elisseeff, Jennifer Hartt.

In: Biomaterials, Vol. 34, No. 37, 12.2013, p. 9365-9372.

Research output: Contribution to journalArticle

Guo, Q, Phillip, JM, Majumdar, S, Wu, PH, Chen, J, Calderón-Colón, X, Schein, OD, Smith, BJ, Trexler, MM, Wirtz, D & Elisseeff, JH 2013, 'Modulation of keratocyte phenotype by collagen fibril nanoarchitecture in membranes for corneal repair', Biomaterials, vol. 34, no. 37, pp. 9365-9372. https://doi.org/10.1016/j.biomaterials.2013.08.061
Guo, Qiongyu ; Phillip, Jude M. ; Majumdar, Shoumyo ; Wu, Pei Hsun ; Chen, Jiansu ; Calderón-Colón, Xiomara ; Schein, Oliver D ; Smith, Barbara J. ; Trexler, Morgana M. ; Wirtz, Denis ; Elisseeff, Jennifer Hartt. / Modulation of keratocyte phenotype by collagen fibril nanoarchitecture in membranes for corneal repair. In: Biomaterials. 2013 ; Vol. 34, No. 37. pp. 9365-9372.
@article{e6ea137ab8e14fcd8474b609eef351b4,
title = "Modulation of keratocyte phenotype by collagen fibril nanoarchitecture in membranes for corneal repair",
abstract = "Type I collagen membranes with tailored fibril nanoarchitectures were fabricated through a vitrification processing, which mimicked, to a degree, the collagen maturation process of corneal stromal extracellular matrix invivo. Vitrification was performed at a controlled temperature of either 5°C or 39°C at a constant relative humidity of 40{\%} for various time periods from 0.5wk up to 8wk. During vitrification, the vitrified collagen membranes (collagen vitrigels, CVs) exhibited a rapid growth in fibrillar density through the evaporation of water and an increase in fibrillar stiffness due to the formation of new and/or more-stable interactions. On the other hand, the collagen fibrils in CVs maintained their D-periodicity and showed no significant difference in fibrillar diameter, indicating preservation of the native states of the collagen fibrils during vitrification. Keratocyte phenotype was maintained on CVs to varying degrees that were strongly influenced by the collagen fibril nanoarchitectures. Specifically, the vitrification time of CVs mainly governed the keratocyte morphology, showing significant increases in the cell protrusion number, protrusion length, and cell size along with CV vitrification time. The CV vitrification temperature affected the regulation of keratocyte fibroblasts' gene expressions, including keratocan and aldehyde dehydrogenase (ALDH), demonstrating a unique way to control the expression of specific genes invitro.",
keywords = "Collagen maturation, Corneal repair, Fibril nanoarchitecture, Keratocyte phenotype",
author = "Qiongyu Guo and Phillip, {Jude M.} and Shoumyo Majumdar and Wu, {Pei Hsun} and Jiansu Chen and Xiomara Calder{\'o}n-Col{\'o}n and Schein, {Oliver D} and Smith, {Barbara J.} and Trexler, {Morgana M.} and Denis Wirtz and Elisseeff, {Jennifer Hartt}",
year = "2013",
month = "12",
doi = "10.1016/j.biomaterials.2013.08.061",
language = "English (US)",
volume = "34",
pages = "9365--9372",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "37",

}

TY - JOUR

T1 - Modulation of keratocyte phenotype by collagen fibril nanoarchitecture in membranes for corneal repair

AU - Guo, Qiongyu

AU - Phillip, Jude M.

AU - Majumdar, Shoumyo

AU - Wu, Pei Hsun

AU - Chen, Jiansu

AU - Calderón-Colón, Xiomara

AU - Schein, Oliver D

AU - Smith, Barbara J.

AU - Trexler, Morgana M.

AU - Wirtz, Denis

AU - Elisseeff, Jennifer Hartt

PY - 2013/12

Y1 - 2013/12

N2 - Type I collagen membranes with tailored fibril nanoarchitectures were fabricated through a vitrification processing, which mimicked, to a degree, the collagen maturation process of corneal stromal extracellular matrix invivo. Vitrification was performed at a controlled temperature of either 5°C or 39°C at a constant relative humidity of 40% for various time periods from 0.5wk up to 8wk. During vitrification, the vitrified collagen membranes (collagen vitrigels, CVs) exhibited a rapid growth in fibrillar density through the evaporation of water and an increase in fibrillar stiffness due to the formation of new and/or more-stable interactions. On the other hand, the collagen fibrils in CVs maintained their D-periodicity and showed no significant difference in fibrillar diameter, indicating preservation of the native states of the collagen fibrils during vitrification. Keratocyte phenotype was maintained on CVs to varying degrees that were strongly influenced by the collagen fibril nanoarchitectures. Specifically, the vitrification time of CVs mainly governed the keratocyte morphology, showing significant increases in the cell protrusion number, protrusion length, and cell size along with CV vitrification time. The CV vitrification temperature affected the regulation of keratocyte fibroblasts' gene expressions, including keratocan and aldehyde dehydrogenase (ALDH), demonstrating a unique way to control the expression of specific genes invitro.

AB - Type I collagen membranes with tailored fibril nanoarchitectures were fabricated through a vitrification processing, which mimicked, to a degree, the collagen maturation process of corneal stromal extracellular matrix invivo. Vitrification was performed at a controlled temperature of either 5°C or 39°C at a constant relative humidity of 40% for various time periods from 0.5wk up to 8wk. During vitrification, the vitrified collagen membranes (collagen vitrigels, CVs) exhibited a rapid growth in fibrillar density through the evaporation of water and an increase in fibrillar stiffness due to the formation of new and/or more-stable interactions. On the other hand, the collagen fibrils in CVs maintained their D-periodicity and showed no significant difference in fibrillar diameter, indicating preservation of the native states of the collagen fibrils during vitrification. Keratocyte phenotype was maintained on CVs to varying degrees that were strongly influenced by the collagen fibril nanoarchitectures. Specifically, the vitrification time of CVs mainly governed the keratocyte morphology, showing significant increases in the cell protrusion number, protrusion length, and cell size along with CV vitrification time. The CV vitrification temperature affected the regulation of keratocyte fibroblasts' gene expressions, including keratocan and aldehyde dehydrogenase (ALDH), demonstrating a unique way to control the expression of specific genes invitro.

KW - Collagen maturation

KW - Corneal repair

KW - Fibril nanoarchitecture

KW - Keratocyte phenotype

UR - http://www.scopus.com/inward/record.url?scp=84884819950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884819950&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2013.08.061

DO - 10.1016/j.biomaterials.2013.08.061

M3 - Article

C2 - 24041426

AN - SCOPUS:84884819950

VL - 34

SP - 9365

EP - 9372

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 37

ER -