Modulation of HLA antigens in response to the binding of epidermal growth factor by A431 cells

Kazuo Hosoi, Dilip S. Kittur, Michael Edidin

Research output: Contribution to journalArticlepeer-review

Abstract

In a previous study [(1984) J. Cell Biol. 98, 725-731] we showed that the level of human MHC, HLA antigens on A431 carcinoma cells is reduced after these cells bind epidermal growth factor (EGF). Here we use flow cytometry to determine the effects of various doses and times of EGF treatment on HLA expression. We then show that the reduction in HLA expression is associated with a reduction in the level of phosphorylation of immunoprecipitable surface HLA antigens, although longer exposure of cells with EGF increased both surface HLA expression and their phosphorylation levels. Lateral diffusion of HLA antigens is lower in EGF-treated than in control cells. The lower diffusion coefficients measured may be causally related to the decreased phosphorylation of HLA antigens.

Original languageEnglish (US)
Pages (from-to)371-377
Number of pages7
JournalFEBS Letters
Volume231
Issue number2
DOIs
StatePublished - Apr 25 1988

Keywords

  • EGF receptor
  • HLA antigen
  • Lateral diffusion
  • Phosphorylation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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