@article{ad262fcf919d4b95b149db015f9e5f42,
title = "Modulation of d-Serine Levels in Brains of Mice Lacking PICK1",
abstract = "Background: d-serine is an endogenous coagonist of the N-methyl-d-aspartate subtype glutamate receptor. Genetic association studies have implicated genes coding for enzymes associated with d-serine metabolism in schizophrenia and bipolar disorder. Methods: Protein expression of serine racemase (SR) and its binding partner, protein interacting with C-kinase (PICK1), were examined by Western blotting in brains from wildtype and PICK1 knockout mice. Levels of d-serine in wildtype and PICK1 mice were also examined by an established high-pressure liquid chromatography protocol. Results: Expression of SR and PICK1 proteins was developmentally regulated. Although no change was observed in the level of SR protein, levels of d-serine were selectively decreased in the forebrain of neonatal PICK1 knockout mice, compared with those in wildtype mice. Conclusions: PICK1 may be involved in the regulation of brain d-serine levels and SR in a spatially and temporally specific manner.",
keywords = "Bipolar disorder, PICK1, d-serine, knockout mice, schizophrenia, serine racemase",
author = "Takatoshi Hikida and Mustafa, {Asif K.} and Kazuhisa Maeda and Kumiko Fujii and Barrow, {Roxanne K.} and Masoumeh Saleh and Huganir, {Richard L.} and Snyder, {Solomon H.} and Kenji Hashimoto and Akira Sawa",
note = "Funding Information: We thank Dr. Pamela Talalay (Johns Hopkins University) for critical reading of this article. We thank Yukiko Y. Lema for preparation of the manuscript and figures. We thank Dr. Yuanxiang Tao (Johns Hopkins University) and Lauren Oby for material support. This work was supported by U.S. Public Health Service Grant No. MH069853, foundation grants from National Alliance for Research on Schizophrenia and Depression, S-R, and Stanley (to AS); U.S. Public Health Service Grant No. MH18501 and Research Scientist Award No. DA00074 (to SHS); National Institutes of Health Grant No. NS036715 and the Howard Hughes Medical Institutes (to RLH); the Ministry of Education, Culture, Sports, Science and Technology, Japan (to KH); Uehara Memorial Foundation Fellowship (to TH); National Research Service Award No. 1 F30 MH074191 -01A2 (to AKM). TH is currently at Osaka Bioscience Institute, Suita, Japan. ",
year = "2008",
month = may,
day = "15",
doi = "10.1016/j.biopsych.2007.09.025",
language = "English (US)",
volume = "63",
pages = "997--1000",
journal = "Biological Psychiatry",
issn = "0006-3223",
publisher = "Elsevier USA",
number = "10",
}