Modified technique for periodic acid-Schiff staining of glycoproteins on agarose-film electrophoretograms

S. M. Trivedi, C. G. Frondoza, R. L. Humphrey

Research output: Contribution to journalArticle

Abstract

We have modified the periodic acid-Schiff staining technique for glycoproteins for use with thin agarose-film electrophoresis membranes. With this procedure, carbohydrate-rich proteins can be detected with negligible background staining and insignificant staining of nonglycoproteins such as albumin and nonglycosylated Bence Jones proteins (free light chains). On the other hand, carbohydrate-rich M components of immunoglobulins M and A in serum and in cerebrospinal fluid from patients with plasma cell dyscrasia are readily detected. The technique is 2- to 3-fold more sensitive than Ponceau S. Glycoproteins in serum and body fluids can be determined as a routine analytical procedure.

Original languageEnglish (US)
Pages (from-to)836-839
Number of pages4
JournalClinical chemistry
Volume29
Issue number5
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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