Modified technique for periodic acid-Schiff staining of glycoproteins on agarose-film electrophoretograms

S. M. Trivedi, C. G. Frondoza, Richard L Humphrey

Research output: Contribution to journalArticle

Abstract

We have modified the periodic acid-Schiff staining technique for glycoproteins for use with thin agarose-film electrophoresis membranes. With this procedure, carbohydrate-rich proteins can be detected with negligible background staining and insignificant staining of nonglycoproteins such as albumin and nonglycosylated Bence Jones proteins (free light chains). On the other hand, carbohydrate-rich M components of immunoglobulins M and A in serum and in cerebrospinal fluid from patients with plasma cell dyscrasia are readily detected. The technique is 2- to 3-fold more sensitive than Ponceau S. Glycoproteins in serum and body fluids can be determined as a routine analytical procedure.

Original languageEnglish (US)
Pages (from-to)836-839
Number of pages4
JournalClinical Chemistry
Volume29
Issue number5
StatePublished - 1983

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Periodic Acid
Sepharose
Glycoproteins
Carbohydrates
Bence Jones Protein
Staining and Labeling
Cerebrospinal fluid
Myeloma Proteins
Body fluids
Electrophoresis
Immunoglobulin A
Immunoglobulin M
Albumins
Paraproteinemias
Body Fluids
Membranes
Serum
Plasmas
Thin films
Cerebrospinal Fluid

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Modified technique for periodic acid-Schiff staining of glycoproteins on agarose-film electrophoretograms. / Trivedi, S. M.; Frondoza, C. G.; Humphrey, Richard L.

In: Clinical Chemistry, Vol. 29, No. 5, 1983, p. 836-839.

Research output: Contribution to journalArticle

Trivedi, S. M. ; Frondoza, C. G. ; Humphrey, Richard L. / Modified technique for periodic acid-Schiff staining of glycoproteins on agarose-film electrophoretograms. In: Clinical Chemistry. 1983 ; Vol. 29, No. 5. pp. 836-839.
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