Modification of Ran GTPpase-activating protein by the small ubiquitin- related modifier SUMO-1 requires Ubc9, an E2-type ubiquitin-conjugating enzyme homologue

Gene W. Lee, Frauke Melchior, Michael J Matunis, Rohit Mahajan, Qingsheng Tian, Paul Anderson

Research output: Contribution to journalArticle

Abstract

Covalent modification of the Ran GTPase-activating protein RanGAP1 with the ubiquitin-related protein SUMO-1 promotes its association with Nup358, a component of the cytoplasmic fibrils emanating from the nuclear pore complex (1, 2). In Xenopus egg extracts, Nup358 can be found in a complex with Ubc9 (3), a structural homologue of the E2-type ubiquitin-conjugating enzymes (UBCs). Here we show that a subset of the human homologue of Ubc9 (HsUbc9) colocalizes with RanGAP1 at the nuclear envelope. HsUbc9 forms thiolester conjugates with recombinant SUMO-1, but not with recombinant ubiquitin, indicating that it is functionally distinct from E2-type UBCs. Finally, HsUbc9 is required for the modification of RanGAP1 by SUMO-1. These results suggest that HsUbc9 is a component of a novel enzymatic cascade that modifies RanGAP1, and possibly other substrates, with SUMO-1.

Original languageEnglish (US)
Pages (from-to)6503-6507
Number of pages5
JournalJournal of Biological Chemistry
Volume273
Issue number11
DOIs
StatePublished - Mar 13 1998
Externally publishedYes

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Small Ubiquitin-Related Modifier Proteins
Ubiquitin-Conjugating Enzymes
Ubiquitin
SUMO-1 Protein
ran GTP-Binding Protein
GTPase-Activating Proteins
Nuclear Pore
Nuclear Envelope
Xenopus
Ovum
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Modification of Ran GTPpase-activating protein by the small ubiquitin- related modifier SUMO-1 requires Ubc9, an E2-type ubiquitin-conjugating enzyme homologue. / Lee, Gene W.; Melchior, Frauke; Matunis, Michael J; Mahajan, Rohit; Tian, Qingsheng; Anderson, Paul.

In: Journal of Biological Chemistry, Vol. 273, No. 11, 13.03.1998, p. 6503-6507.

Research output: Contribution to journalArticle

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AB - Covalent modification of the Ran GTPase-activating protein RanGAP1 with the ubiquitin-related protein SUMO-1 promotes its association with Nup358, a component of the cytoplasmic fibrils emanating from the nuclear pore complex (1, 2). In Xenopus egg extracts, Nup358 can be found in a complex with Ubc9 (3), a structural homologue of the E2-type ubiquitin-conjugating enzymes (UBCs). Here we show that a subset of the human homologue of Ubc9 (HsUbc9) colocalizes with RanGAP1 at the nuclear envelope. HsUbc9 forms thiolester conjugates with recombinant SUMO-1, but not with recombinant ubiquitin, indicating that it is functionally distinct from E2-type UBCs. Finally, HsUbc9 is required for the modification of RanGAP1 by SUMO-1. These results suggest that HsUbc9 is a component of a novel enzymatic cascade that modifies RanGAP1, and possibly other substrates, with SUMO-1.

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